A new PCR-based approach for the specific amplification of DNA from different Schistosoma species applicable to human urine samples
A new PCR-based approach for the specific amplification of DNA from different Schistosoma species applicable to human urine samples
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DOI：
10.1017/s0031182006000898
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发表时间：
2006-11-01
期刊：
影响因子：
2.4
通讯作者：
Muro, A.
中科院分区：
文献类型：
--
作者：
Sandoval, N.;Siles-Lucas, M.;Muro, A.
目前用于诊断人血吸虫病的方法往往缺乏足够的敏感性和特异性。最近，一些作者开发了更特异和敏感的诊断方法，主要是基于聚合酶链反应（PCR）技术。然而，这些方法仅用于诊断影响人类的4种血吸虫中的1种（S。mansoni）。此外，特异性PCR的应用专门用于血液或粪便患者的样品。在这里，我们开发了一种新的，高灵敏度的PCR方法，允许属和种特异性扩增的主要4种血吸虫引起的疾病，在人加上S。牛。我们进一步成功地应用这种技术检测寄生虫DNA在易于处理的尿样本血吸虫病患者。使用这些样本，我们发现当应用属特异性（血吸虫属）时，灵敏度为94.4%，特异性为99.9%。特异性为98.9%，敏感性为100%。mansoni）PCR。
Currently available methods for the diagnosis of human schistosomiasis often lack enough sensitivity and specificity. Recently, several authors have developed more specific and sensitive diagnostic methods, mainly based on the polymerase chain reaction (PCR) technique. Nevertheless, these have been only applied for the diagnosis of 1 out of 4 Schistosoma species affecting man (S. mansoni). Additionally, application of specific PCR has been exclusively used for blood or faecal patients' samples. Here, we develop a new, high sensitive PCR approach that allows the genus- and species-specific amplification of the main 4 Schistosoma species causing disease in man plus S. bovis. We further successfully apply this technique for the detection of parasite DNA in easy-to-handle urine samples from patients with schistosomiasis. With these samples, we have found 94.4% sensitivity and 99.9% specificity when applying a genus-specific (Schistosoma spp.) primer pair, and 100%, sensitivity and 98.9% specificity in a species-specific (S. mansoni) PCR.