Physiological Characteristics of Melon Plants Showing Leaf Yellowing Symptoms Caused by CABYV Infection
Physiological Characteristics of Melon Plants Showing Leaf Yellowing Symptoms Caused by CABYV Infection
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DOI：
10.7235/hort.2015.14149
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发表时间：
2015-04-01
影响因子：
--
通讯作者：
Chun, Changhoo
中科院分区：
文献类型：
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作者：
Lee, Hee Ju;Kim, Mi-Kyeong;Chun, Changhoo
利用电子显微镜和RT-PCR对韩国报道的主要感染葫芦的病毒（CMV、MNSV、CGMMV、SqMV、WMV、KGMMV、PRSV和ZYMV）进行了分析，但没有检测到这些病毒。通过下一代测序（NGS）进一步分析，确定该病毒为瓜类蚜传黄化病毒（CABYV），并利用CABYV特异性引物进行RT-PCR鉴定。当根据叶绿素荧光产量（ChlFY）测量光合能力时，患病植物的叶片显示4.09 μ mol.m（-2）.s（-1），这是未受影响的正常植物观察到的读数（12.36 μ mol.m（-2）.s（-1））的三分之一。受叶片黄化症状（LYS）影响的植物的根功能为0.28 mg.g（-1），约为正常未受影响植物（0.48 mg.g（-1））的一半。细胞学观察表明，病株叶片的栅栏薄壁组织和叶肉海绵细胞与正常植株无形态学差异。然而，与正常的未受影响的植物相比，受影响植物的相同叶细胞含有更多的淀粉粒。因此，甜瓜的叶枯病不仅是一种生理性病害，而且是一种由CABYV引起并由蚜虫传播的病毒性病害。
Melon leaves showing yellowing symptoms were analyzed using electron microscopy and RT-PCR for major cucurbit-infecting-viruses (CMV, MNSV, CGMMV, SqMV, WMV, KGMMV, PRSV and ZYMV) reported in Korea, but these viruses were not detected. As the result of further analysis by next-generation sequencing (NGS), the virus was identified as Cucurbit aphid-borne yellows virus (CABYV), and then confirmed by RT-PCR using CABYV-specific primers. When photosynthetic capacity was measured based on chlorophyll fluorescence yield (ChlFY), the leaves of the diseased plants showed 4.09 mu mol.m(-2).s(-1), which was one-third of the readings observed for unaffected normal plants (12.36 mu mol.m(-2).s(-1)). The root functions of plants affected by leaf yellowing symptoms (LYS) was 0.28 mg.g(-1), about half that measured for the normal unaffected plants (0.48 mg.g(-1)). Cytological observations revealed that there were no morphological differences in the palisade parenchyma and mesophyll spongy cells of the leaves between the diseased and the normal plants. However, the same leaf cells of the affected plants contained more starch granules compared to those of the normal, unaffected plants. We conclude that the LYS of muskmelon is not merely a physiological disorder but a viral disease caused by CABYV and spread by aphids.