Plasma FFA utilization and fatty acid-binding protein content are diminished in type 2 diabetic muscle
Plasma FFA utilization and fatty acid-binding protein content are diminished in type 2 diabetic muscle
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DOI：
10.1152/ajpendo.2000.279.1.e146
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发表时间：
2000-07-01
影响因子：
5.1
通讯作者：
Saris, WHM
中科院分区：
文献类型：
--
作者：
Blaak, EE;Wagenmakers, AJM;Saris, WHM
在这项研究中，我们研究了这样的假设：2 型糖尿病患者在隔夜禁食状态和 β 肾上腺素能刺激期间都存在前臂骨骼肌游离脂肪酸 (FFA) 代谢损伤。八名患有 2 型糖尿病的肥胖受试者和八名非肥胖对照者 (Con) 在禁食过夜后输注稳定同位素示踪剂 [U-C-13] 棕榈酸酯期间和输注非选择性 β 激动剂异丙肾上腺素（Iso，20 ng·kg 去脂体重 (-1)·min(-1)）期间，使用前臂平衡技术和间接量热法进行了研究。此外，在股外侧肌活检中测定了线粒体酶和细胞质脂肪酸结合蛋白（FABP）的活性。在禁食和 Iso 输注期间，示踪剂平衡数据显示前臂肌肉 FFA 摄取（Con 与类型 2：快速 449 +/- 69 与 258 +/- 42 和 Iso 715 +/- 129 与 398 +/- 70 nmol。100 ml 组织(-1) . min(-1)，P< 0.05）和 FFA 释放在类型中较低2型糖尿病与Con相比。此外，在 2 型糖尿病患者输注 Iso 期间，骨骼肌对血浆 FFA 的氧化减弱（Con 与 2 型：Iso 446 +/- 274 对比 16 +/- 70 nmol。100 ml 组织(-1) . min(-1)，P < 0.05）。 2 型糖尿病受试者的前臂甘油净释放量增加（P < 0.05），这表明前臂脂肪分解增加。此外，2 型糖尿病患者骨骼肌细胞质 FABP 含量和肌肉氧化酶活性降低。我们得出的结论是，在有或没有 Iso 刺激的过夜禁食状态下，2 型糖尿病受试者的前臂肌肉中血浆 FFA 的摄取和氧化均受到损害。
In this study, we investigated the hypothesis that impairments in forearm skeletal muscle free fatty acid (FFA) metabolism are present in patients with type 2 diabetes both in the overnight fasted state and during beta-adrenergic stimulation. Eight obese subjects with type 2 diabetes and eight nonobese controls (Con) were studied using the forearm balance technique and indirect calorimetry during infusion of the stable isotope tracer [U-C-13] palmitate after an overnight fast and during infusion of the nonselective beta-agonist isoprenaline (Iso, 20 ng . kg lean body mass(-1) . min(-1)). Additionally, activities of mitochondrial enzymes and of cytoplasmatic fatty acid-binding protein (FABP) were determined in biopsies from the vastus lateralis muscle. Both during fasting and Iso infusion, the tracer balance data showed that forearm muscle FFA uptake (Con vs. type 2: fast 449 +/- 69 vs. 258 +/- 42 and Iso 715 +/- 129 vs. 398 +/- 70 nmol . 100 ml tissue(-1) . min(-1), P< 0.05) and FFA release were lower in type 2 diabetes compared with Con. Also, the oxidation of plasma FFA by skeletal muscle was blunted during Iso infusion in type 2 diabetes (Con vs. type 2: Iso 446 +/- 274 vs. 16 +/- 70 nmol . 100 ml tissue(-1) . min(-1), P< 0.05). The net forearm glycerol release was increased in type 2 diabetic subjects (P< 0.05), which points to an increased forearm lipolysis. Additionally, skeletal muscle cytoplasmatic FABP content and the activity of muscle oxidative enzymes were lowered in type 2 diabetes. We conclude that the uptake and oxidation of plasma FFA are impaired in the forearm muscles of type 2 diabetic subjects in the overnight fasted state with and without Iso stimulation.