Molecular cloning of the cDNAs encoding two gonadotropin beta subunits (GTH-I beta and -II beta) from the goldfish, Carassius auratus.
Molecular cloning of the cDNAs encoding two gonadotropin beta subunits (GTH-I beta and -II beta) from the goldfish, Carassius auratus.
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编码来自金鱼鲫鱼的两个促性腺激素 β 亚基（GTH-I β 和 -II β）的 cDNA 的分子克隆。
DOI：
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发表时间：
1997
影响因子：
2.7
通讯作者：
K. Aida
中科院分区：
文献类型：
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作者：
Y. Yoshiura;M. Kobayashi;Y. Kato;K. Aida
从金鱼垂体cDNA文库中克隆了两种编码促性腺激素β亚基的cDNA （GTH-I β和-II β）。GTH-I β cDNA全长616 bp，编码130个氨基酸；GTH-II β cDNA全长552 bp，编码140个氨基酸。这两种类型的金鱼GTH β亚基在核苷酸序列和氨基酸序列上的一致性分别为52%和38%。与哺乳动物GTH β亚基相比，金鱼GTH- 1 β与fsh - β s的同源性高于lh - β s，金鱼GTH- 2 β与lh - β s的同源性高于lh - β s。基因组Southern blot分析显示，鲤科鱼类中含有与金鱼GTH- 1 β和-II β同源的基因，这表明鲤科鱼类中GTH的双重性与其他硬骨鱼中一样。Northern blot分析不同卵巢成熟阶段（未成熟、成熟、成熟和退化）金鱼的GTH-I β和-II β mRNA水平显示，GTH-I β和-II β mRNA水平随成熟的进展而升高，在退化阶段下降。这些结果表明，金鱼的性腺发育受两种GTHs的调控。
Two types of cDNAs (GTH-I beta and -II beta) that encode the beta subunit of goldfish gonadotropin were cloned from a goldfish pituitary cDNA library. The nucleotide sequence of GTH-I beta cDNA was 616 bp long, encoding 130 amino acids, and that of GTH-II beta was 552 bp long, encoding 140 amino acids. These two types of goldfish GTH beta subunits showed an identity of 52% in the nucleotide sequence and 38% in the amino acid sequence. When compared to mammalian GTH beta subunits, goldfish GTH-I beta showed higher homology to FSH-beta s than LH-beta s, and goldfish-II beta to LH-beta s. Genomic Southern blot analysis revealed that cyprinid species harbored genes which were homologous to goldfish GTH-I beta and -II beta, demonstrating the duality of GTH in cyprinid fish as has been shown in other teleost fish. Northern blot analysis of GTH-I beta and -II beta mRNAs in goldfish at differing stages of ovarian maturity (immature, maturing, mature, and regressed) showed that both GTH-I beta and -II beta mRNA levels increased with the progression of maturity and declined in regressed stage. These results suggested that gonadal development in the goldfish was regulated by the two GTHs.