Acupuncture ameliorated skeletal muscle atrophy induced by hindlimb suspension in mice
Acupuncture ameliorated skeletal muscle atrophy induced by hindlimb suspension in mice
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DOI：
10.1016/j.bbrc.2011.05.152
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发表时间：
2011-07-08
影响因子：
3.1
通讯作者：
Fukubayashi, Toru
中科院分区：
文献类型：
--
作者：
Onda, Akiko;Jiao, Qibin;Fukubayashi, Toru
预防骨骼肌萎缩对于维持生活质量至关重要，但对于老年人和病情严重的患者来说，这往往是一个具有挑战性的目标。我们假设针灸代替运动训练是一种替代的非药物干预，可以帮助预防肌肉萎缩。为探讨针刺对后肢悬吊（HS）所致骨骼肌萎缩的影响，采用电针和非电针两种方法对小鼠进行针刺治疗。EA组和MA组每天在腓肠肌内留针30 min，共2周。电针组同时给予频率1Hz、脉宽1 ms、强度6.5mA的重复电刺激30 min。HS显着减少肌肉质量和横截面积的比目鱼肌。这种HS诱导的减少在EA组中得到显著改善，尽管与对照组相比，改善的水平仍然不足。我们发现，atrogin-1和MuRF 1的mRNA表达水平，这在肌肉特异性降解E3泛素连接酶中发挥主要作用，显着增加HS组相比，对照组。EA和MA减少HS诱导的atrogin-1（EA和MA中p < 0.01）和MuRF 1（EA中p < 0.01）mRNA的上调。我们还发现，PI 3 K，Akt 1，TRPV 4，腺苷A1受体，肌肉生长抑制素和SIRT 1 mRNA的表达水平有增加的趋势。EA和MA进一步增加HS诱导的Akt 1（MA中p < 0.05）和TRPV 4（MA中p < 0.05）mRNA的上调。我们的结论是，针灸部分防止骨骼肌萎缩。这种效应可能是由于蛋白质合成的增加和蛋白质降解的减少。(C)2011 Elsevier Inc. All rights reserved.
Preventing skeletal muscle atrophy is critical for maintaining quality of life, but it is often a challenging goal for the elderly and patients with severe conditions. We hypothesized that acupuncture in place of exercise training is an alternative non-pharmacological intervention that can help to prevent muscle atrophy. To elucidate the effects of acupuncture on skeletal muscle atrophy caused by hindlimb suspension (HS), we performed acupuncture on mice according to two different methods: acupuncture with electrical stimulation (EA: electroacupuncture) and without electrical stimulation (MA: manual acupuncture). A needle was retained in the gastrocnemius muscle for 30 min every day for 2 weeks in the EA and MA groups. In the EA group, 30 min of repetitive electrical stimulation (1 Hz, 1 ms pulse width, 6.5 m]A intensity) was also applied. HS significantly reduced muscle mass and the cross-sectional area of the soleus muscles. This HS-induced reduction was significantly improved in the EA group, although the level of improvement remained insufficient when compared with the control group. We found that the mRNA expression levels of atrogin-1 and MuRF1, which play a principal role in muscle-specific degradation as E3 ubiquitin ligases, were significantly increased in the HS group compared to the control group. EA and MA reduced the HS-induced upregulation of atrogin-1 (p < 0.01 in EA and MA) and MuRF1 (p < 0.01 in EA) mRNAs. We also found that the expression levels of PI3K, Akt1, TRPV4, adenosine A1 receptor, myostatin, and SIRT1 mRNAs tended to be increased by HS. EA and MA further increased the HS-induced upregulation of Akt1 (p < 0.05 in MA) and TRPV4 (p < 0.05 in MA) mRNAs. We concluded that acupuncture partially prevented skeletal muscle atrophy. This effect might be due to an increase in protein synthesis and a decrease in protein degradation. (C) 2011 Elsevier Inc. All rights reserved.