Synthesis of functional eukaryotic ribosomal RNAs in trans: Development of a novel in vivo rDNA system for dissecting ribosome biogenesis
Synthesis of functional eukaryotic ribosomal RNAs in trans: Development of a novel in vivo rDNA system for dissecting ribosome biogenesis
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DOI：
10.1073/pnas.94.7.2864
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发表时间：
1997-04-01
影响因子：
11.1
通讯作者：
Fournier, MJ
中科院分区：
文献类型：
--
作者：
Liang, WQ;Fournier, MJ
活性18 S和25 S核糖体RNA在酵母中反式产生，从含有RNA聚合酶II转录信号和具有独特杂交标签的rDNA片段的质粒。用温度敏感的RNA聚合酶I在细胞中进行分析。功能性rRNA分别来自18 S和5.8/25 SrRNA编码单位，而活性25 SrRNA只能通过与5.8SrRNA共转录产生。结果表明，大rDNA操纵子的多顺反子结构并不是成功加工I-RN、I或组装功能性核糖体所必需的。
Active 18S and 25S ribosomal RNAs were produced in trans in yeast, from plasmids containing RNA polymerasc II transcription signals and rDNA fragments with unique hybridization tags. Analyses were carried out in cells with temperature-sensitive RNA polymerase I. Functional rRNAs were derived from separate 18S and 5.8/25S rRNA coding units, however, active 25S rRNA could be produced only by cotranscription with 5.8S rRNA. The results demonstrate that the polycistronic organization of the large rDNA operon is not required for successful processing of I-RN,I or assembly of functional ribosomes, The split operon system should facilitate future efforts to dissect eukaryotic ribosome biogenesis.