Inducible Prrxl1-CreERT2 recombination activity in the somatosensory afferent pathway
Inducible Prrxl1-CreERT2 recombination activity in the somatosensory afferent pathway
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体感传入通路中可诱导的 Prrxl1-CreERT2 重组活性
DOI：
10.1002/dvg.22020
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发表时间：
2012-07-01
期刊：
影响因子：
1.5
通讯作者：
Ding, Yu-Qiang
中科院分区：
文献类型：
--
作者：
Hu, Ze-Lan;Huang, Ying;Ding, Yu-Qiang
通过修饰含有Prrxl 1的BAC克隆产生表达他莫昔芬诱导型Cre重组酶的Prrxl 1-CreERT 2转基因小鼠。在Prrxl 1-CreERT 2; Rosa 26报告小鼠的不同胚胎和出生后阶段检查Cre重组活性。妊娠小鼠在胚胎第9.5或E12.5天用单剂量的他莫昔芬处理，并在2天后进行X-gal染色。在躯体感觉神经节中观察到强X-gal染色（例如，背根和三叉神经节）和用于处理躯体感觉信息的第一中枢部位（例如，脊髓背角和三叉神经相关核）。当他莫昔芬在出生后第20天（P）或在成年期（P120），强Cre重组活动存在于初级躯体感觉神经节，而弱Cre重组活动被发现在脊髓背角，中脑三叉神经核，三叉神经主感觉核，三叉神经脊束核。这种小鼠品系为以时间控制的方式探索基因在躯体感觉系统中的功能提供了有用的工具。Genesis 50：552560，2012. (c)2012 Wiley Periodicals，Inc.
Prrxl1-CreERT2 transgenic mice expressing tamoxifen-inducible Cre recombinase were generated by modifying a Prrxl1-containing BAC clone. Cre recombination activity was examined in Prrxl1-CreERT2; Rosa26 reporter mice at various embryonic and postnatal stages. Pregnant mice were treated with a single dose of tamoxifen at embryonic day (E) 9.5 or E12.5, and X-gal staining was performed 2 days later. Strong X-gal staining was observed in the somatosensory ganglia (e.g., dorsal root and trigeminal ganglia) and the first central sites for processing somatosensory information (e.g., spinal dorsal horn and trigeminal nerve-associated nuclei). When tamoxifen was administered at postnatal day (P) 20 or in adulthood (P120), strong Cre recombination activity was present in the primary somatosensory ganglia, while weak Cre recombination activity was found in the spinal dorsal horn, mesencephalic trigeminal nucleus, principal sensory trigeminal nucleus, and spinal trigeminal nucleus. This mouse line provides a useful tool for exploring genes' functions in the somatosensory system in a time-controlled way. genesis 50:552560, 2012. (c) 2012 Wiley Periodicals, Inc.