Two types of VIP neuronal components in rat suprachiasmatic nucleus
Two types of VIP neuronal components in rat suprachiasmatic nucleus
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DOI：
10.1002/jnr.10751
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发表时间：
2003-12-15
影响因子：
4.2
通讯作者：
Okamura, H
中科院分区：
文献类型：
--
作者：
Kawamoto, K;Nagano, M;Okamura, H
血管活性肠肽（VIP）神经元在视交叉上核（SCN）中构成一个大群，被认为参与昼夜节律的产生和携带。我们对大鼠SCN中这些表达VIP的神经元进行了表征，通过它们在光照下诱导哺乳动物周期1 （Per1）基因的能力、视网膜传入神经的支配、VIP mRNA的昼夜变化以及胃泌素释放肽（GRP）的共表达。将腹侧SCNVL的VIP神经元分为光诱发的per1诱导型主SCNVL （SCNVLmain）和非per1诱导型中层SCNVL （SCNVLmed）两组。视网膜神经支配在中央区丰富，而中央区几乎没有。在SCNVLmain中观察到VIP mRNA表达水平的昼夜变化，而在SCNVLmed中没有。GRP mRNA很少出现在SCNVLmed中，但在SCNVLmain中大量出现，其中一些神经元共表达VIP mRNA。这些发现表明，SCN中的VIP神经元可以分为两组不同的地形和功能。(C) 2003 Wiley-Liss, Inc。
Vasoactive intestinal peptide (VIP) neurons constitute a large group in the suprachiasmatic nucleus (SCN) and it is thought that they are involved in the generation and entrainment of circadian rhythm. We have characterized these VIP-expressing neurons in rat SCN by their ability to induce the mammalian Period1 (Per1) gene in response to light exposure, innervation of retinal afferents, day-night variations in VIP mRNA, and coexpression of gastrin releasing peptide (GRP). VIP neurons in the ventrolateral SCN (SCNVL) were subdivided into two groups, light-evoked Per1-inducible main SCNVL (SCNVLmain) and non-Per1-inducible medially located SCNVL (SCNVLmed). Retinal innervation was abundant in the SCNVLmain but nearly absent in the SCNVLmed. Day-night variation in VIP mRNA expression level was observed in the SCNVLmain but not in the SCNVLmed. GRP mRNA was seen in rarely SCNVLmed but abundant in SCNVLmain, where some neurons coexpressed VIP mRNA. These findings indicate that VIP neurons in the SCN can be divided into two topographically and functionally distinct groups. (C) 2003 Wiley-Liss, Inc.