A Novel Antifungal Plasma-Activated Hydrogel.
A Novel Antifungal Plasma-Activated Hydrogel.
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DOI：
10.1021/acsami.9b04700
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发表时间：
2019-06
影响因子：
9.5
通讯作者：
Zhengxin Liu;Yongping Zheng;J. Dang;Jiabin Zhang;Feihong Dong;Kaile Wang;Jue Zhang
中科院分区：
文献类型：
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作者：
Zhengxin Liu;Yongping Zheng;J. Dang;Jiabin Zhang;Feihong Dong;Kaile Wang;Jue Zhang
添加抗真菌药物的抗真菌水凝胶由于其在伤口敷料和超声凝胶垫等各种应用中的潜在用途而受到研究人员的广泛关注。在这项研究中，我们提出并设计了一种替代的抗真菌水凝胶制备策略，以获得具有高抗真菌能力的水凝胶。我们采用等离子体活化水（PAW）代替水在水凝胶聚合过程中制备等离子体活化水凝胶（PAH）。纸片扩散法结果表明，PAH具有良好的抗真菌活性。有趣的是，氧化还原电位（ORP）的PAH是显着低于传统的聚丙烯酰胺（PAAm）水凝胶，我们提供了一个可能的反应方程来解释较低的值在PAH的氧化还原电位（ORP）。此外，使用电子自旋共振（ESR）光谱，羟基自由基检测等离子体激活的水凝胶（PAH）。虽然水凝胶中的活性成分无法定量测定，但根据ESR光谱和光发射光谱推测羟基自由基和NO3-是具有抗真菌活性的PAH的主要成分。进一步的实验还表明，PAH比PAW具有更长的抗真菌寿命。综上所述，所提出的等离子体活化水凝胶可以提供有价值的制备策略，提供抗真菌能力，并在生物医学领域有许多潜在的应用。
Antifungal hydrogels with added antifungal drugs have received extensive attention from researchers due to their potential use in various applications, such as wound dressings and ultrasound gel pads. In this study, we proposed and designed an alternative antifungal hydrogel preparation strategy to obtain hydrogels with high antifungal abilities. We employed plasma-activated water (PAW) instead of water in the hydrogel polymerization process to prepare plasma-activated hydrogels (PAH). Disc diffusion assay results revealed that PAH exhibits satisfactory antifungal activity. Interestingly, the oxidation-reduction potential (ORP) of the PAH was significantly lower than that of conventional polyacrylamide (PAAm) hydrogels, and we provided a possible reaction equation to explain the lower value of ORP in the PAH. Furthermore, using electron spin resonance (ESR) spectroscopy, the hydroxyl radical was detected in plasma-activated hydrogels (PAH). Although the active ingredients in the hydrogel cannot be quantitatively measured, the hydroxyl radical and NO3- are speculated to be the main components of PAH with antifungal activity, according to ESR spectroscopy and optical emission spectroscopy. Further experiments also showed that PAH has a longer antifungal lifetime than PAW. In summary, the proposed plasma-activated hydrogels can provide valuable preparation strategies for delivering antifungal capabilities and have many potential applications in biomedical fields.