Ligand-Functionalization of BPEI-Coated YVO4:Bi3+,Eu3+ Nanophosphors for Tumor-Cell-Targeted Imaging Applications
Ligand-Functionalization of BPEI-Coated YVO4:Bi3+,Eu3+ Nanophosphors for Tumor-Cell-Targeted Imaging Applications
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DOI：
10.1002/asia.201300570
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发表时间：
2013-11-01
影响因子：
4.1
通讯作者：
Chen, Teng-Ming
中科院分区：
文献类型：
--
作者：
Chen, Yi-Chin;Huang, Sheng-Cih;Chen, Teng-Ming
本研究采用一种简单、快速、无溶剂的水热法制备了表面功能化的支化聚乙烯亚胺(BPEI)修饰的YVO4：Bi3+，Eu3+纳米晶。结晶度较高的BPEI包覆的YVO4：Bi3+，Eu3+纳米管在=250~400 nm的近紫外区有宽带激发，在=350 nm的激发下有一个位于=619 nm的尖峰发射带。封端剂BPEI贡献的表面氨基不仅改善了包覆BPEI的YVO4：Bi3+，Eu3+纳米颗粒的分散性和水/缓冲稳定性，还提供了一种特定靶向生物分子连接的能力。叶酸(FA)和表皮生长因子(EGF)进一步结合在BPEI包被的YVO4：Bi3+，Eu3+NCS上，并显示出荧光NCS对HeLa细胞中高表达的叶酸受体或A431细胞中高表达的EGFR的有效定位，且细胞毒性较低。这些结果表明，配体功能化、BPEI包覆的YVO4：Bi3+，Eu3+纳米管作为新一代生物发光生物探针在生物成像领域具有巨大的潜力。此外，BPEI包覆的YVO4：Bi3+，Eu3+纳米管独特的发光特性显示了与UVA光敏药物结合产生检测和治疗人类皮肤癌治疗效果的潜力。
In this study, surface-functionalized, branched polyethylenimine (BPEI)-modified YVO4:Bi3+,Eu3+ nanocrystals (NCs) were successfully synthesized by a simple, rapid, solvent-free hydrothermal method. The BPEI-coated YVO4:Bi3+,Eu3+ NCs with high crystallinity show broad-band excitation in the =250 to 400nm near-ultraviolet (NUV) region and exhibit a sharp-line emission band centered at =619nm under excitation at =350nm. The surface amino groups contributed by the capping agent, BPEI, not only improve the dispersibility and water/buffer stability of the BPEI-coated YVO4:Bi3+,Eu3+ NCs, but also provide a capability for specifically targeted biomolecule conjugation. Folic acid (FA) and epidermal growth factor (EGF) were further attached to the BPEI-coated YVO4:Bi3+,Eu3+ NCs and exhibited effective positioning of fluorescent NCs toward the targeted folate receptor overexpressed in HeLa cells or EGFR overexpressed in A431 cells with low cytotoxicity. These results demonstrate that the ligand-functionalized, BPEI-coated YVO4:Bi3+, Eu3+ NCs show great potential as a new-generation biological luminescent bioprobe for bioimaging applications. Moreover, the unique luminescence properties of BPEI-coated YVO4:Bi3+,Eu3+ NCs show potential to combine with a UVA photosensitizing drug to produce both detective and therapeutic effects for human skin cancer therapy.