Identification and characterization of a novel gene family YPEL in a wide spectrum of eukaryotic species
Identification and characterization of a novel gene family YPEL in a wide spectrum of eukaryotic species
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DOI：
10.1016/j.gene.2004.06.014
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发表时间：
2004-09-29
期刊：
影响因子：
3.5
通讯作者：
Shimizu, N
中科院分区：
文献类型：
--
作者：
Hosono, K;Sasaki, T;Shimizu, N
通过对人类22号染色体的综合序列分析，我们发现了一个与果蝇yippee基因高度同源的新基因家族（YPEL1 ~ YPEL5）。我们克隆并测序了所有五个基因的cdna，并确定了它们的外显子/内含子组织。这些YPEL基因在氨基酸序列水平上具有较高的同源性（43.8% ~ 96.6%）。小鼠同源基因（Ypel1至Ypel5）也在小鼠染色体的syntenic区被鉴定出来，并对其cdna进行了克隆和测序。5对人/鼠同源物均显示出极高的同源性。因此，我们将这些基因命名为YPEL基因家族的成员。我们从公共数据库中检索了YPEL家族基因，发现了来自68个物种的100个基因，包括动物、植物和真菌。这100个YPEL蛋白的氨基酸序列非常相似，所有YPEL家族蛋白无一例外都建立了c - x -2- c - x -19- g - x -3- l - x -5- n - x -13- g - x -8- c -2- c - x -4- gwxy - x -10- k - x -6- e的一致序列。有趣的是，间接免疫荧光染色表明，在COS-7细胞的有丝分裂期，YPEL 1-4蛋白定位于中心体和核核，并在有丝分裂期位于有丝分裂器周围的几个点状结构上。在同一细胞系的有丝分裂过程中，YPEL5蛋白定位于间期的中心体和细胞核以及有丝分裂纺锤体。因此，YPEL家族蛋白基本上存在于所有真核生物中，因此它们必须在维持生命中发挥重要作用。与中心体或有丝分裂纺锤体相关的YPEL蛋白的亚细胞定位提示细胞分裂中涉及一种新的功能。(C) 2004 Elsevier B.V.版权所有
During comprehensive sequence analysis of human chromosome 22, we identified a novel gene family consisting of five members (YPEL1 through YPEL5) which has high homology with Drosophila yippee gene. We cloned and sequenced cDNAs for all five genes and determined their exon/intron organization. These YPEL genes showed high homology (43.8-96.6%) at amino acid sequence level among them. Mouse counterparts (Ypel1 through Ypel5) were also identified in the syntenic region of mouse chromosomes and their cDNAs were cloned and sequenced. Each of five pairs of human/mouse orthologs revealed extremely high homology. Thus, we named these genes as members of YPEL gene family. We searched YPEL family genes from the public databases, and found 100 genes from 68 species including animals, plants and fungi. Amino acid sequences of these 100 YPEL proteins were extremely similar and a consensus sequence of C-X-2-C-X-19-G-X-3-L-X-5-N-X-13-G-X-8-C-X-2-C-X-4-GWXY-X-10-K-X-6-E was established for all the YPEL family proteins without exception. Interestingly, the indirect immunofluorescent staining indicated that YPEL 1-4 proteins are localized to the centrosome and nucleolus during interphase and at several dot-like structures around the mitotic apparatus during mitotic phase of COS-7 cells. YPEL5 protein is localized to the centrosome and nucleus during interphase and at the mitotic spindle during mitosis of the same cell line. Thus, the YPEL family proteins were found in essentially all the eukaryotes and hence they must play important roles in the maintenance of life. The subcellular localization of YPEL proteins in association with centrosome or mitotic spindle suggests a novel function involved in the cell division. (C) 2004 Elsevier B.V. All rights reserved.