CHEMICAL-PROPERTIES, DISTRIBUTION, AND PHYSIOLOGY OF PLANT AND ALGAL CARBONIC-ANHYDRASES
CHEMICAL-PROPERTIES, DISTRIBUTION, AND PHYSIOLOGY OF PLANT AND ALGAL CARBONIC-ANHYDRASES
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DOI：
10.1111/j.1749-6632.1984.tb12340.x
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发表时间：
1984-01-01
影响因子：
5.2
通讯作者：
DWYER, MR
中科院分区：
文献类型：
--
作者：
GRAHAM, D;REED, ML;DWYER, MR
植物碳酸酐酶(CA)具有一定的相对分子质量(MW)。在开花植物中，C3光合作用的双子叶植物有两个140-250K的同工酶，每个有6个亚基，而单子叶植物有两个42-45K的同工酶。植物和动物CA具有相似的氨基酸含量、亚基大小和锌含量，表明它们是同源蛋白质，尽管高等植物CA没有酯酶活性，也不受磺胺类化合物的强烈抑制。藻类CAs的相对分子质量变化很大，有些对磺胺类药物高度敏感，如动物酶。植物的两种同工酶，来自叶绿体和细胞质，可以通过梯度聚丙烯酰胺凝胶电泳法分离，然后通过酶促H+离子的产生来可视化。在植物中，CA可能有助于二氧化碳向光合作用固定部位的扩散；它们还可能在调节pH、水生植物利用碳酸氢盐以及在叶绿体中浓缩无机碳方面发挥作用。
Plant carbonic anhydrases (CAs) have a range of molecular weights (MW). Among flowering plants, dicotyledons with C3 photosynthesis have two isoenzymes of 140-250K each with 6 subunits, while monocotyledons have two isoenzymes of 42-45K. Plant and animal CAs have a similar amino acid content, subunit size and zinc content, suggesting they are homologous proteins, although the higher plant CAs have no esterase activity and are not strongly inhibited by sulfonamides. Algal CAs vary widely in MW and some are highly sensitive to sulfonamides like the animal enzymes. The two plant isoenzymes, from the chloroplast and cytosol, can be separated by gradient polyacrylamide gel electrophoresis and subsequently visualized by enzymic H+ ion production. In plants, CAs probably facilitate diffusion of CO2 to the site of photosynthetic fixation; they may also have a role in pH regulation, in the use of bicarbonate by aquatic plants and in concentrating inorganic carbon within the chloroplast.