Vestibularly evoked climbing-fiber responses modulate simple spikes in rabbit cerebellar Purkinje neurons.
Vestibularly evoked climbing-fiber responses modulate simple spikes in rabbit cerebellar Purkinje neurons.
复制标题
前庭诱发的攀爬纤维反应调节兔小脑浦肯野神经元的简单尖峰。
DOI：
10.1111/j.1749-6632.2002.tb07571.x
复制
发表时间：
2002
影响因子：
5.2
通讯作者：
Yakhnitsa,Vadim
中科院分区：
文献类型：
--
作者：
Barmack,NealH;Yakhnitsa,Vadim
摘要：脑结节接受来自同侧迷路的初级前庭传入神经输入和来自对侧迷路的与前庭相关的攀爬纤维输入。先前我们证明了结节性浦肯野细胞中前庭诱发的攀爬纤维反应（CFRs）放电的增加与简单尖峰放电（SSs）的减少相关。前庭诱发的CFRs和SSs的反相行为是否反映了一种共同的神经机制，还是两个独立的平行通路的激活，这一问题尚未解决。我们用自然前庭刺激来调节单侧迷路切除、氯氯脲麻醉的家兔小舌结节浦肯野细胞的细胞外放电，回答了这个问题。在这些动物中，在单侧迷路切除术的对侧，以苔藓状纤维形式投射到小舌结节的前庭初级传入神经保持完整。在同侧结节性浦肯野细胞中，同侧侧侧侧倾斜增加了CFRs的放电，而对侧侧侧倾斜增加了SSs的放电。这些极性在对侧小舌结节记录的浦肯野细胞中被逆转。从结节两侧浦肯野细胞记录的SS放电极性与前庭初级苔藓纤维传入的极性相反。即使初级前庭传入苔藓纤维通路因单侧迷路切除而被破坏，SSs仍对对侧侧滚动倾斜有反应。虽然在对侧小舌结节中记录到的SSs放电随着对侧侧侧倾斜而增加，但相对于在同侧小舌结节中记录的浦肯野细胞中观察到的这种调节减少。我们认为，前庭诱发的CFRs引起了SS放电的调节。
Abstract:The nodulus receives a primary vestibular afferent input from the ipsilateral labyrinth and a vestibularly related climbing‐fiber input originating from the contralateral labyrinth. Previously we demonstrated that increased discharge of vestibularly evoked climbing‐fiber responses (CFRs) in nodular Purkinje cells was correlated with decreased discharge of simple spikes (SSs). This left unresolved the question of whether vestibularly evoked antiphasic behavior of CFRs and SSs reflects a common neural mechanism or the activation of two separate parallel pathways. We answered this question using natural vestibular stimulation to modulate the discharge of uvula‐nodular Purkinje cells recorded extracellularly in unilaterally labyrinthectomized, chloralose urethane‐anesthetized rabbits. In such animals, vestibular primary afferents projecting to the uvula‐nodulus as mossy fibers remained intact on the side contralateral to the unilateral labyrinthectomy. The discharge of CFRs recorded in ipsilateral nodular Purkinje cells was increased by ipsilateral roll‐tilt while the discharge of SSs was increased by contralateral roll‐tilt. These polarities were reversed for Purkinje cells recorded in the contralateral uvula‐nodulus. The polarity of SS discharge recorded from Purkinje cells on both sides of the nodulus was opposite to that of the vestibular primary mossy‐fiber afferents. SSs continued to respond to contralateral roll‐tilt even when the primary vestibular afferent mossy‐fiber pathway was destroyed by the unilateral labyrinthectomy. Although the discharge of SSs recorded in the contralateral uvula‐nodulus was increased by contralateral roll‐tilt, this modulation was reduced relative to that observed in Purkinje cells recorded in the ipsilateral uvula‐nodulus. We conclude that vestibularly evoked CFRs caused the modulation of SS discharge.