Genomic cloning and complete sequence analysis of a highly divergent African human immunodeficiency virus isolate
Genomic cloning and complete sequence analysis of a highly divergent African human immunodeficiency virus isolate
复制标题
高度分化的非洲人类免疫缺陷病毒分离株的基因组克隆和完整序列分析
DOI：
10.1128/jvi.68.3.1586-1596.1994
复制
发表时间：
1994
影响因子：
5.4
通讯作者：
B. Gent
中科院分区：
文献类型：
--
作者：
'. J. Decourt;R. J. Leys;Guido;van der;Groen;Hugo;Van;Heuverswijn;B. Gent
从喀麦隆患者身上获得的人类免疫缺陷病毒（HIV）分离物（Ant70）的完整序列分析表明，这种病毒是迄今为止所描述的HIV-1家族中分化程度最高的毒株。Pol蛋白通常在HIV-1家族中高度保守，与hiv分离物的相似性仅为73%，而对于包膜等更可变的蛋白质，相似性为50%或更低。gp41的主要中和决定因子（V3环）和免疫优势区也包含一些不寻常的取代，这可能对蛋白质功能以及基于这些区域的血清学分析有影响。系统发育分析表明，该分离物相对于人类HIV-1分离物和最近描述的SIVcpz病毒具有独特的位置，表明该喀麦隆分离物可能为我们提供有关HIV-1家族起源的新见解。
Analysis of the complete sequence of a human immunodeficiency virus (HIV) isolate (Ant70) obtained from a Cameroonian patient indicates that this virus is the most divergent strain within the HIV-1 family hitherto described. Comparison of the Pol protein, usually highly conserved within the HIV-1 family, shows only about 73% similarity with the HIVmm isolate, whereas for the more variable proteins such as envelope, similarities of 50% or lower are found. The principal neutralizing determinant (V3 loop) and the immunodominant region within gp41 also contain some unusual substitutions, which may have implications for protein function as well as for serological assays based on these regions. Phylogenetic analyses show that this isolate occupies a unique position relative to the human HIV-1 isolates and the recently described SIVcpz virus, indicating that this Cameroonian isolate may provide us with new insights into the origins of the HIV-1 family.
DOI：
10.1073/pnas.87.2.523
发表时间：
1990-01-01
影响因子：
11.1
作者：
BRYANT, M;RATNER, L
通讯作者：
RATNER, L