A genetic, deletion, physical, and human homology map of the long fin region on zebrafish linkage group 2
A genetic, deletion, physical, and human homology map of the long fin region on zebrafish linkage group 2
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DOI：
10.1006/geno.2002.6769
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发表时间：
2002-06-01
期刊：
影响因子：
4.4
通讯作者：
Johnson, SL
中科院分区：
文献类型：
--
作者：
Iovine, MK;Johnson, SL
长鳍基因突变导致斑马鱼鳍过度生长。因此，长鳍基因的分子鉴定可能揭示正常生长控制的机制。因此，我们开发了连锁群 2 (LG2) 相应区域的遗传和物理图谱。还产生了长鳍基因的单个缺失等位基因（lof(jg6l)）。检查该缺失是否存在独立映射到 LG2 的 EST，发现 SSLP、STS 和基于基因的标记的连续缺失跨越约 500 kb 的物理距离，包括至少 10 个斑马鱼基因。斑马鱼长鳍区域基因的人类直向同源物被鉴定出来，并揭示了来自人类 1 号染色体 (Hsa1) 和 Hsa19 的两个同线性片段。对与人类直系同源物相关的其他基因的同源性搜索揭示了长鳍缺失区域中的一个额外基因。因此，我们开发的长鳍区域的遗传、物理、缺失和人类同源图谱提供了斑马鱼区域与同源人类区域的第一个高分辨率比较之一，并促进了长鳍基因的分子鉴定。
Mutation of the gene long fin causes overgrowth of zebrafish fins. Thus, molecular identification of the gene long fin may reveal the mechanisms involved in normal growth control. We have therefore developed genetic and physical maps of the corresponding region on linkage group 2 (LG2). A single deletion allele (lof(jg6l)) of the long fin gene was also generated. Examination of this deletion for the presence or absence of ESTs independently mapped to LG2 revealed a contiguous deletion of SSLP, STS, and gene-based markers spanning a physical distance of approximately 500 kb, including at least 10 zebrafish genes. Human orthologs of the zebrafish genes in the long fin region were identified and revealed two synteny segments from human chromosome 1 (Hsa1) and Hsa19. Homology searching for additional genes linked to the human orthologs revealed one additional gene in the long fin deletion region. Thus, our development of the genetic, physical, deletion, and human homology maps of the long fin region provides one of the first high-resolution comparisons of a zebrafish region with a homologous human region, and facilitates the molecular identification of the long fin gene.