Dopamine physiology in the basal ganglia of male zebra finches during social stimulation.
Dopamine physiology in the basal ganglia of male zebra finches during social stimulation.
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DOI：
10.1111/ejn.12887
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发表时间：
2015-06
期刊：
影响因子：
--
通讯作者：
Doupe AJ
中科院分区：
文献类型：
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作者：
Ihle EC;van der Hart M;Jongsma M;Tecott LH;Doupe AJ
越来越多的证据表明，多巴胺(DA)参与了斑马雀皮层-基底神经节中专门负责唱歌的神经活动和基因表达的改变，在求爱过程中从单独唱歌到唱歌。我们的目标是采样成年和幼年鸟类X区细胞外DA浓度的变化，以检验这两个年龄组的DA水平在呈现社会显著刺激的过程中变化相似的假设。采用微透析法对清醒、行为正常的成年和幼年雄性斑马雀X区细胞外环境进行采样，并采用高效液相色谱-电化学检测相结合的方法对透析液进行分析。在雌性呈现给成年男性和家教呈现给幼年男性的过程中，X区DA的胞外水平显著增加。DA水平与唱歌时间无关。我们还用直接作用于DA系统或作用于去甲肾上腺素的药理学药物对X区进行了反向透析，发现所有这些药物都显著增加了X区的DA水平(3-10倍)。这些发现表明，细胞外DA水平的变化可以类似地受到非常不同的社会环境(求爱和与导师的互动)的刺激，并受到多巴胺和去甲肾上腺素能药物的强烈影响。这些结果提出了这样一种可能性，即受试者的唤醒水平或注意力状态(而不是歌唱行为)是引起细胞外DA浓度变化的共同特征。
Accumulating evidence suggests that dopamine (DA) is involved in altering neural activity and gene expression in a zebra finch cortical–basal ganglia circuit specialized for singing, upon the shift between solitary singing and singing as a part of courtship. Our objective here was to sample changes in the extracellular concentrations of DA in Area X of adult and juvenile birds, to test the hypothesis that DA levels would change similarly during presentation of a socially salient stimulus in both age groups. We used microdialysis to sample the extracellular milieu of Area X in awake, behaving adult and juvenile male zebra finches, and analysed the dialysate using high-performance liquid chromatography coupled with electrochemical detection. The extracellular levels of DA in Area X increased significantly during both female presentation to adult males and tutor presentation to juvenile males. DA levels were not correlated with the time spent singing. We also reverse-dialysed Area X with pharmacologic agents that act either on DA systems directly or on norepinephrine, and found that all of these agents significantly increased DA levels (3- to 10-fold) in Area X. These findings suggest that changes in extracellular DA levels can be stimulated similarly by very different social contexts (courtship and interaction with tutor), and influenced potently by dopaminergic and noradrenergic drugs. These results raise the possibility that the arousal level or attentional state of the subject (rather than singing behavior) is the common feature eliciting changes in extracellular DA concentration.