SOME FACTORS INFLUENCING VITAMIN-B12 PRODUCTION BY PSEUDOMONAS-DENITRIFICANS
SOME FACTORS INFLUENCING VITAMIN-B12 PRODUCTION BY PSEUDOMONAS-DENITRIFICANS
复制标题
DOI：
10.1139/m70-136
复制
发表时间：
1970-01-01
影响因子：
2.8
通讯作者：
DANIELS, HJ
中科院分区：
文献类型：
--
作者：
DANIELS, HJ
在由蔗糖、谷氨酸和无机盐组成的化学成分确定的基础培养基中，研究了已知的前体和辅因子对维生素B12产生的影响。只有碳源甜菜碱和Co2+被发现是维生素B12生产所必需的。已知的前体如蛋氨酸、δ-氨基乙酰丙酸、琥珀酸和1-氨基-2-丙醇没有影响。甘氨酸对生长有抑制作用。草酸、乳酸、5，6-二甲基苯并咪唑和Mo ~（2+）对维生素B_（12）的生物合成有促进作用。特别令人感兴趣的是草酸，其在0.04%w/v的水平下使维生素产量增加约20%，并且用洗涤的细胞可以取代谷氨酸，谷氨酸已被证明对生长和维生素B12的生产都是必需的。 当使用草酸盐时，发现这种酸在快速生长和维生素产生期间消失，然后在发酵周期的最后阶段重新出现。
The effect of known precursors and cofactors on vitamin B12production has been studied in a basal, chemically defined medium composed of sucrose, glutamic acid, and mineral salts. Only a carbon source, betaine, and Co2+were found to be essential for vitamin B12production. Known precursors such as methionine, δ-aminolevulinic acid, succinic acid, and 1-amino-2-propanol had no effect. Glycine was inhibitory to growth. Oxalic acid, lactic acid, 5,6-dimethylbenzimidazole, and Mo2+stimulated vitamin B12biosynthesis. Of special interest is oxalic acid, which at a level of 0.04% w/v increased vitamin production about 20%, and with washed cells could replace glutamic acid, which had been demonstrated to be essential for both growth and vitamin B12production. When use of oxalate was followed, it was found that this acid disappeared during the period of rapid growth and vitamin production and then reappeared during the final stages of the fermentation cycle.