Redox status-mediated regulation of gene expression and virulence in the brown rot pathogen Monilinia fructicola
Redox status-mediated regulation of gene expression and virulence in the brown rot pathogen Monilinia fructicola
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DOI：
10.1111/ppa.12006
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发表时间：
2013-08-01
期刊：
影响因子：
2.7
通讯作者：
Lee, M. -H.
中科院分区：
文献类型：
--
作者：
Chiu, C. -M.;You, B. -J.;Lee, M. -H.
MfCUT 1基因编码的主要角质酶是核果类水果开花枯萎病和果腐病的病原菌的致病因子。病原体保持静止的阶段II水果，其中含有高水平的绿原酸，咖啡酸（CA）的奎尼酸酯。建立了一个培养基转换系统，以显示MfCUT 1表达被CA下调，与之前在连续培养中的发现一致，并且被抗氧化剂谷胱甘肽（GSH）、N-乙酰基-L-半胱氨酸和抗坏血酸下调。然而，MfCUT 1的表达是由超氧化物生成氧化剂甲萘醌和谷胱甘肽合成抑制剂丁硫氨酸磺酰亚胺（BSO）上调。CA或BSO诱导的MfCUT 1转录水平的变化与细胞内GSH和H2 O2水平有关。此外，CA还能提高GSH还原酶的活性。克隆了编码可能有助于氧化还原稳态（GSH还原酶和GSH过氧化物酶）的酶的桃褐腐病菌基因，发现它们的表达在培养物暴露于CA后受到轻微影响。杂交茶树品种。火焰和平被确定为替代宿主，以补充核果类水果上的致病性测定，这是季节性限制。离体桃果实和玫瑰花瓣的致病性试验表明，CA抑制和H2 O2增强褐腐病的形成。结果表明，细胞氧化还原状态的变化影响MfCUT 1的表达和毒力的果生分枝杆菌，并建议GSH的氧化还原循环与这种调节。
The MfCUT1 gene encoding the major cutinase of Monilinia fructicola, a causal agent of blossom blight and fruit rot of stone fruits, is a virulence factor of the pathogen. The pathogen remains quiescent on stage II fruit, which contain high levels of chlorogenic acid, a quinate ester of caffeic acid (CA). A medium shift system was established to show that MfCUT1 expression is down-regulated by CA, consistent with previous findings in continuous culture, and by the antioxidants glutathione (GSH), N-acetyl-l-cysteine and ascorbic acid. However, MfCUT1 expression is up-regulated by the superoxide-generating oxidant menadione and by the GSH synthesis inhibitor buthionine sulphoximine (BSO). The changes in MfCUT1 transcript levels induced by CA or BSO are related to the levels of intracellular GSH and H2O2. In addition, GSH reductase activity was increased by CA. Monilinia fructicola genes encoding enzymes that may contribute to redox homeostasis (GSH reductase and GSH peroxidase) were cloned and their expression was found to be slightly affected following exposure of cultures to CA. The hybrid tea rose cv. Flaming Peace was identified as an alternative host to complement pathogenicity assays of M.fructicola on stone fruits, which are seasonally restricted. Pathogenicity assays on detached peach fruit and rose petals revealed that CA suppresses and H2O2 enhances formation of brown rot lesions. The results indicate that changes in cellular redox status impact MfCUT1 expression and virulence in M.fructicola, and suggest that redox cycling of GSH is related to this regulation.