Lysosomes in lymphoid tissue. I. The measurement of hydrolytic activities in whole homogenates.
Lysosomes in lymphoid tissue. I. The measurement of hydrolytic activities in whole homogenates.
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淋巴组织中的溶酶体。
DOI：
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发表时间：
1967
期刊：
影响因子：
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通讯作者：
C. Duve
中科院分区：
文献类型：
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作者：
W. Bowers;J. Finkenstaedt;C. Duve
建立了大鼠脾匀浆中细胞色素氧化酶、酯酶和11种酸性水解酶的定量测定方法。这些方法似乎也适用于其他淋巴组织。初步研究，延伸到9个酸水解酶，表明这些酶发生在部分潜伏和sedimentable形式，它们可以被揭露和呈现可溶性的一些治疗释放的酶从大鼠肝溶酶体。脾脏颗粒似乎对机械损伤非常敏感，这是一种需要在组织中采取特殊预防措施的特性。脾颗粒的凝集发生在0.25 M蔗糖比在0.15 M KCl更大的程度。
Methods have been developed for the quantitative assay of cytochrome oxidase, esterase, and 11 acid hydrolases in rat-spleen homogenates. These methods seem to be applicable also to other lymphoid tissues. Preliminary studies, extended to nine of the acid hydrolases, indicate that these enzymes occur in partly latent and sedimentable form and that they can be unmasked and rendered soluble by some of the treatments that liberate the enzymes from rat-liver lysosomes. The spleen particles appear to be very sensitive to mechanical injury, a property which necessitates special precautions in homogenizing the tissue. Agglutination of spleen particles takes place to a larger extent in 0.25 M sucrose than in 0.15 M KCl.