PSI:Biology-materials repository: a biologist's resource for protein expression plasmids
PSI:Biology-materials repository: a biologist's resource for protein expression plasmids
复制标题
DOI：
10.1007/s10969-011-9100-8
复制
发表时间：
2011-07-01
期刊：
影响因子：
--
通讯作者：
LaBaer, Joshua
中科院分区：
文献类型：
--
作者：
Cormier, Catherine Y.;Park, Jin G.;LaBaer, Joshua
蛋白质结构倡议：生物材料储存库(PSI：Biology-MR；MR；http://psimr.asu.edu)Sequence)-验证、注释、存储和分发由蛋白质结构倡议创建的蛋白质表达质粒和载体。MR已经开发了一条信息学和样品处理管道，每月管理来自近12个PSI中心的数千个样品。DNASU(http://dnasu.asu.edu)，是一个可免费搜索的数据库，存储了由本实验室、PSI和其他联盟以及各个实验室创建的130,000多个质粒的全长序列、载体信息和相关出版物，以分发给世界各地的研究人员。每个质粒都链接到外部资源，包括PSI结构生物学知识库(http://sbkb.org)，)，该知识库有助于将特定的质粒交叉引用到额外的蛋白质注释和实验数据。为了加快和简化质粒请求，MR使用快速材料转移协议(EP-MTA)网络，来自网络机构的研究人员可以在没有机构延迟的情况下订购和接收PSI质粒。截至2011年3月，应DNASU的要求，PSI提供了超过39,000个蛋白质表达质粒和78个空载体。总体而言，MR的表达准备好的质粒库，其自动化管道，以及更有效地接收和分发这些质粒的快速过程，使研究界能够剖析其结构已被PSI研究的蛋白质的生物学功能。
The Protein Structure Initiative:Biology-Materials Repository (PSI:Biology-MR; MR; http://psimr.asu.edu) sequence-verifies, annotates, stores, and distributes the protein expression plasmids and vectors created by the Protein Structure Initiative (PSI). The MR has developed an informatics and sample processing pipeline that manages this process for thousands of samples per month from nearly a dozen PSI centers. DNASU (http://dnasu.asu.edu), a freely searchable database, stores the plasmid annotations, which include the full-length sequence, vector information, and associated publications for over 130,000 plasmids created by our laboratory, by the PSI and other consortia, and by individual laboratories for distribution to researchers worldwide. Each plasmid links to external resources, including the PSI Structural Biology Knowledgebase (http://sbkb.org), which facilitates cross-referencing of a particular plasmid to additional protein annotations and experimental data. To expedite and simplify plasmid requests, the MR uses an expedited material transfer agreement (EP-MTA) network, where researchers from network institutions can order and receive PSI plasmids without institutional delays. As of March 2011, over 39,000 protein expression plasmids and 78 empty vectors from the PSI are available upon request from DNASU. Overall, the MR's repository of expression-ready plasmids, its automated pipeline, and the rapid process for receiving and distributing these plasmids more effectively allows the research community to dissect the biological function of proteins whose structures have been studied by the PSI.