Myosin 1D and the branched actin network control the condensation of p62 bodies
Myosin 1D and the branched actin network control the condensation of p62 bodies
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DOI：
10.1038/s41422-022-00662-6
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Mi Na
中科院分区：
文献类型：
--
作者：
Feng Xuezhao;Du Wanqing;Ding Mingrui;Zhao Wenkang;Xirefu Xirenayi;Ma Meisheng;Zhuang Yuhui;Fu Xiaoyu;Shen Jiangfeng;Zhang Jinpei;Lei Xiuying;Sun Daxiao;Xi Qing;Aisa Yiliyasi;Chen Qian;Li Ying;Wang Wenjuan;Huang Shanjin;Yu Li;Li Pilong;Mi Na
Biomolecular condensation driven by liquid–liquid phase separation (LLPS) is key to assembly of membraneless organelles in numerous crucial pathways. It is largely unknown how cellular structures or components spatiotemporally regulate LLPS and condensate formation. Here we reveal that cytoskeletal dynamics can control the condensation of p62 bodies comprising the autophagic adaptor p62/SQSTM1 and poly-ubiquitinated cargos. Branched actin networks are associated with p62 bodies and are required for their condensation. Myosin 1D, a branched actin-associated motor protein, drives coalescence of small nanoscale p62 bodies into large micron-scale condensates along the branched actin network. Impairment of actin cytoskeletal networks compromises the condensation of p62 bodies and retards substrate degradation by autophagy in both cellular models and Myosin 1D knockout mice. Coupling of LLPS scaffold to cytoskeleton systems may represent a general mechanism by which cells exert spatiotemporal control over phase condensation processes.
