Type-I interferon regulates matrix metalloproteinases clearance of the bovine endometrial spheroid
Type-I interferon regulates matrix metalloproteinases clearance of the bovine endometrial spheroid
复制标题
I 型干扰素调节牛子宫内膜球体的基质金属蛋白酶清除
DOI：
10.1111/asj.13350
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发表时间：
2020
影响因子：
2
通讯作者：
Nobuhiko Yamauchi
中科院分区：
文献类型：
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作者：
Al-Nur Md. Iftekhar Rahman;Seiya Yamashita;Md. Rashedul Islam;Taisuke Fujihara;Hayato Yamaguchi;Manabu Kawahara;Masashi Takahashi;Hideyuki Takahashi;Takafumi Gotoh;Nobuhiko Yamauchi
本研究探讨了I型干扰素对牛子宫内膜间质细胞(BES)和上皮细胞(BEE)基质金属蛋白酶(MMPs)表达的影响。从肉瘤中分离纯化细胞，在含有10%胎牛血清的DMEM/F-12中培养。用抗坏血酸生成球体。培养上清液的酶谱显示，蜜蜂主要表达MMP9，而MMP2在BES和均球体中表达。而在异形球体中未检测到MMPs的表达。实时定量聚合酶链式反应显示，I型干扰素和P4分别抑制异种球体中基质金属蛋白酶-2和基质金属蛋白酶-9的基因表达。明胶酶谱分析表明，I型干扰素对MMPs的清除有很强的促进作用。而I型干扰素可显著降低异种球体中MMPs的酶谱。苯甲基磺酰氟和亮肽素(均为丝氨酸蛋白酶抑制剂)显著抑制I型干扰素诱导的基质金属蛋白酶-2和基质金属蛋白酶-9的清除。此外，经I型干扰素处理后，异种球体中的胶原纤维明显减少。提示I型干扰素可能通过调节MMPs的清除参与组织重塑。
This study investigated the effect of type‐I interferon (IFN) on the expression of matrix metalloproteinases (MMPs) of the bovine endometrial stromal cells (BES) and epithelial cells (BEE). The cells were separated and purified from the caruncles and cultured in DMEM/F‐12 containing 10% fetal bovine serum. Spheroids were generated by using ascorbate. Zymograms of the supernatant showed that BEE predominantly expressed MMP‐9, whereas MMP‐2 was expressed in BES and homo‐spheroids. While MMPs expression was not detected in hetero‐spheroids. Real‐time quantitative PCR revealed that type‐I IFN and P4 suppressed the gene expression of MMP‐2 and MMP‐9 in hetero‐spheroids, respectively. On the other hand, gelatin zymography analysis of the supernatant showed that type‐I IFN strongly promote the clearance of MMPs. While zymograms of the MMPs stocked in the hetero‐spheroids were significantly reduced by type‐I IFN. Phenylmethanesulfonyl fluoride and leupeptin (both are serine proteinase inhibitors) significantly repressed the clearance of MMP‐2 and MMP‐9 induced by type‐I IFN. Moreover, collagen fibers in hetero‐spheroids significantly decreased after the treatment with type‐I IFN. In conclusion, it was suggested that type‐I IFN participate in the tissue remodeling by regulation the clearance of MMPs.