Detection of nanoparticles in a minute sample using the vibration induced flow
Detection of nanoparticles in a minute sample using the vibration induced flow
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DOI：
10.1109/nems54180.2022.9791090
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发表时间：
2022-04
期刊：
影响因子：
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通讯作者：
K. Kaneko;M. Tsugane;Taku Sato;T. Hayakawa;Y. Hasegawa;H. Suzuki
中科院分区：
文献类型：
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作者：
K. Kaneko;M. Tsugane;Taku Sato;T. Hayakawa;Y. Hasegawa;H. Suzuki
我们开发了一种振动诱导流动(VIF)微流控检测纳米颗粒的系统，该系统通过施加小的周期性振动在微柱周围诱导平均流动。在该系统中，通过VIF搅拌的亲和捕获微珠的聚集程度来评估样品流体中特定NPs的存在。在这里，我们表明，样品中的NPs数量与在短时间(~10分钟)内形成的捕获珠团聚体的平均面积有很好的相关性，这一点可以很容易地通过亮场显微镜成像进行评估。我们还表明，聚集的程度取决于振动参数，并且存在一个最佳条件，利用VIF可以更快地检测到NPs。此外，我们使用我们的系统成功地检测了细胞外小泡(EVS)，以检验其在实际应用中的适用性。该方法简便、重现性好，可作为一种通用、可靠的NPs样品检测方法。
We developed a microfluidic system for the detection of nanoparticles (NPs) with the vibration-induced flow (VIF), in which a mean flow is induced around a micro-pillar by applying the small periodical vibration. In this system, the presence of specific NPs in a sample fluid is evaluated by the extent of the aggregation of the affinity capture beads stirred by VIF. Here, we show that the amount of NPs in the sample correlates well with the average area of the capture bead aggregates formed within a short time (~10 min), which can be readily evaluated by the bright field microscope imaging. We also show that the extent of the aggregation depends on vibration parameters, and there is an optimal condition to detect NPs faster using VIF. Further, we succeeded in detecting the extracellular vesicles (EVs) using our system, for examination its applicability in real application. This method is simple and reproducible, so that it can be used as a versatile and reliable detection method of NPs in a minute sample.