Role of a Viral Membrane Polypeptide in Strand-Specific Initiation of Poliovirus RNA Synthesis
Role of a Viral Membrane Polypeptide in Strand-Specific Initiation of Poliovirus RNA Synthesis
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DOI：
10.1128/jvi.65.7.3972-3972.1991
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发表时间：
1991-07
影响因子：
5.4
通讯作者：
Cristina Giachetti;B. Semler
中科院分区：
文献类型：
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作者：
Cristina Giachetti;B. Semler
分子遗传分析与体外生化方法相结合，以确定脊髓灰质炎病毒RNA合成过程中核苷酸蛋白形成所需的功能相互作用。对病毒膜蛋白疏水域的定点损伤产生了一种突变病毒，它在39℃时RNA合成存在缺陷。这种损伤的表型表达影响RNA合成的启动，基因组连接蛋白(VPG)的体外尿苷化，以及体内正链病毒RNA的合成。我们的结果支持使用病毒膜蛋白作为VPG的载体来启动正链RNA合成的模型。我们的数据还表明，一种单独的机制可以用于启动负链RNA合成，从而为微小RNA病毒复制的链特异性调控提供一种手段。
A molecular genetic analysis has been combined with an in vitro biochemical approach to define the functional interactions required for nucleotidyl protein formation during poliovirus RNA synthesis. A site-directed lesion into the hydrophobic domain of a viral membrane protein produced a mutant virus that is defective in RNA synthesis at 39 degrees C. The phenotypic expression of this lesion affects initiation of RNA synthesis, in vitro uridylylation of the genome-linked protein (VPg), and the in vivo synthesis of plus-strand viral RNAs. Our results support a model that employs a viral membrane protein as carrier for VPg in the initiation of plus-strand RNA synthesis. Our data also suggest that a separate mechanism could be used in the initiation of minus-strand RNA synthesis, thereby providing a means for strand-specific regulation of picornavirus RNA replication.