Effect of high-pressure microfluidization treatment on the physicochemical properties and antioxidant activities of polysaccharide from Mesona chinensis Benth
Effect of high-pressure microfluidization treatment on the physicochemical properties and antioxidant activities of polysaccharide from Mesona chinensis Benth
复制标题
高压微流化处理对凉粉草多糖理化性质及抗氧化活性的影响
DOI：
10.1016/j.carbpol.2018.07.087
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发表时间：
2018-11-15
影响因子：
11.2
通讯作者：
Xie, Jianhua
中科院分区：
文献类型：
--
作者：
Huang, Lixin;Shen, Mingyue;Xie, Jianhua
动态高压似乎是对多糖进行物理修饰以改善其功能特性的一种替代方法。本研究旨在评价动态高压微流态化(DHPM)处理对仙人掌多糖(MP)的理化性质和抗氧化活性的影响。结果表明，经DHPM处理后，多糖样品中总糖和糖醛酸的含量显著增加，蛋白质含量显著降低。MP和DMP的相对分子质量分别为1.58×10(5)Da和1.64×10(5)Da。MP和DMP由Gal、Xyl和Gala组成，摩尔比分别为2.8：5.5：2.4和3.8：7.4：3.0。经DHPM处理后，MP的抗氧化活性略有提高。此外，DHPM处理还会导致多糖的形态发生变化。扫描电子显微镜分析表明，经DHPM处理的多糖样品的表面形貌与原始多糖相比略有卷曲。FT-IR分析表明，MP和DMP之间的结构没有明显的变化。这些结果为MP的进一步应用提供了有用的信息，并表明DHPM处理可以提高多糖的抗氧化活性。
Dynamic high-pressure appears to be an alternative approach to physical modification of polysaccharides aimed to improve their functional characteristics. The objective of our study was to evaluate the effect of dynamic high-pressure microfluidization (DHPM) treatment on the physicochemical properties and antioxidant activities of Mesona chinensis Benth polysaccharide (MP). The results indicated that the contents of total sugar and uronic acid in DHPM-treated polysaccharide samples (DMP) were increased, and protein content in DMP was significantly decreased after DHPM treatment. The molecular weights of MP and DMP were 1.58x10(5) Da and 1.64x10(5) Da. MP and DMP were composed of Gal, Xyl, and GalA in a molar ratio of 2.8: 5.5: 2.4 and 3.8: 7.4: 3.0, respectively. The antioxidant activities of MP were slightly promoted after DHPM treatment. Moreover, DHPM treatment leads to changes in the morphology of polysaccharide. The surface appearances of DHPM-treated polysaccharide samples showed a slightly curly surface compared to original polysaccharide by SEM analysis. No considerable changes were observed in the structure between the MP and DMP by FT-IR. The results provide useful information for future application of MP, and show DHPM treatment can improve the antioxidant activity of polysaccharides.