FIXATION IN CYTOCHEMISTRY AND ELECTRON-MICROSCOPY
FIXATION IN CYTOCHEMISTRY AND ELECTRON-MICROSCOPY
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DOI：
10.1177/6.5.303
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发表时间：
1958-01-01
影响因子：
3.2
通讯作者：
BAKER, JR
中科院分区：
文献类型：
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作者：
BAKER, JR
作者敦促采用更批判性的方法来解释电子显微照片，并敦促细胞化学家和电子显微镜学家之间进行更密切的合作。他认为，除非可以独立检查，否则应该对亚微观水平上揭示的结构持怀疑态度。例如，人们可以利用特定物质的已知趋势以特定方式排列自身。因此，磷脂具有以平行或同心层的形式存在的强烈倾向。这些层可能已经存在于生命中，但必须记住，它们最初是脂蛋白复合物的一部分，通过固定剂的“揭开”作用而分离，然后才采用层状排列。
The author urges a more critical approach to the interpretation of electron-micrographs, and closer collaboration between cytochemists and electron-microscopists. He considers that there should be scepticism about structure revealed at the sub-microscopic level, unless it can be checked independently. For instance, one may make use of the known tendency of particular substances to arrange themselves in particular ways. Thus, phospholipids have a strong tendency to present themselves in the form of parallel or concentric layers. These layers may already have been present in life, but the possibility must be borne in mind that they originally formed part of lipoprotein complexes, were separated off by the "unmasking" effect of the fixative, and only then adopted a lamellar arrangement.