Estrogen potentiates the combined effects of transforming growth factor-beta and tumor necrosis factor-alpha on adult human osteoblast-like cell prostaglandin E2 biosynthesis.
Estrogen potentiates the combined effects of transforming growth factor-beta and tumor necrosis factor-alpha on adult human osteoblast-like cell prostaglandin E2 biosynthesis.
复制标题
雌激素增强转化生长因子-β 和肿瘤坏死因子-α 对成人成骨细胞样细胞前列腺素 E2 生物合成的联合作用。
DOI：
10.1007/s00223-002-0023-z
复制
发表时间：
2003
影响因子：
4.2
通讯作者：
Keeting,PE
中科院分区：
文献类型：
--
作者：
Secreto,FJ;Grover,A;Pacurari,M;Rice,MB;Kantorow,M;Bidwai,AP;Blaha,JD;Keeting,PE
雌激素治疗通过骨和骨细胞调节花生四烯酸代谢的报道见于文献。然而，从这些报告的分析中，出现了雌激素和花生四烯酸代谢之间存在的关系的相互矛盾的迹象。本研究进行，以确定是否雌激素的影响，在人成骨细胞样（hOB）细胞培养衍生自成人，在基础或姜黄素刺激的条件下，产生的前列腺素（PG）。48小时雌激素预处理没有改变hOB细胞PG生物合成的定性基础上，和PGE 2的形成占主导地位，在所有测试条件下。雌激素预处理可导致转化生长因子-β 1和肿瘤坏死因子-α联合刺激的标本中PGE 2的产生增加（p< 0.001）。在雌激素预处理的标本中，单独用两种测试的细胞因子刺激，也没有观察到PGE 2产生的变化，在TGFβ或TNF被白细胞介素-1 β替代的标本中也没有观察到PGE 2产生的变化。抗雌激素（ICI 164，384）的加入阻止了TGFβ + TNF刺激样品中PGE 2产生的雌激素依赖性增加。这些结果表明，雌激素对骨细胞前列腺素生物合成的影响可能是最明显和显着的条件下，细胞暴露于多种骨细胞因子，骨重建过程中的条件。
Reports that estrogen treatment modulates arachidonic acid metabolism by bone and bone cells are found in the literature. However, conflicting indications of the relationship that exists between estrogen and arachidonic acid metabolism emerge from the analysis of those reports. The present studies were undertaken to determine if estrogen effected the production of prostaglandins (PG) in human osteoblast-like (hOB) cell cultures derived from adults, under basal or cytokine-stimulated conditions. A 48-hour estrogen pretreatment did not modify hOB cell PG biosynthesis on a qualitative basis, and PGE2formation predominated under all tested conditions. Estrogen pretreatment did lead to increased PGE2production in specimens stimulated conjointly with transforming growth factor-β1and tumor necrosis factor-α (p< 0.001). No changes in PGE2production were observed in estrogen pretreated specimens stimulated singly with either tested cytokine, nor in samples in which either TGFβ or TNF was replaced by interleukin-1β. Anti-estrogen (ICI 164,384) inclusion prevented the estrogen-dependent increase in PGE2production in the TGFβ plus TNF-stimulated samples. These results suggest that an estrogen effect on bone cell prostaglandin biosynthesis may be most evident and significant under conditions in which the cells are exposed to multiple osteotropic cytokines, a condition that applies during the bone remodeling process.