Roles of three Fusarium graminearum membrane Ca2+ channels in the formation of Ca2+ signatures, growth, development, pathogenicity and mycotoxin production
Roles of three Fusarium graminearum membrane Ca2+ channels in the formation of Ca2+ signatures, growth, development, pathogenicity and mycotoxin production
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DOI：
10.1016/j.fgb.2017.11.005
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发表时间：
2018-02-01
影响因子：
3
通讯作者：
Kang, Seogchan
中科院分区：
文献类型：
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作者：
Kim, Hye-Seon;Kim, Jung-Eun;Kang, Seogchan
与动植物一样，外界刺激也会引起真菌胞质Ca ~（2+）的时空动态变化。这种变化被称为Ca 2+信号，通过调节不同Ca 2+结合蛋白（CBP）的活性或位置以及间接影响与CBP相互作用的蛋白质来控制细胞反应。因此，为了理解Ca 2+信号传导的基础机制，表征Ca 2+如何在不同条件下进出细胞质以产生Ca 2+信号是至关重要的。对禾谷镰刀菌（Fusarium graminearum）细胞质中表达基于荧光蛋白的Ca 2+指示剂的质膜Ca 2+通道的3个编码基因进行诱变处理，以研究它们在不同生长条件和遗传背景下产生Ca 2+信号的作用。被破坏的基因包括编码高亲和力Ca 2+摄取系统的CCH 1和FIG 1，以及编码低亲和力Ca 2+通道的FIG 1。还分析了所得突变体的生长、发育、致病性和真菌毒素产生，以确定每个基因的缺失如何改变这些性状。为了研究单个基因是否影响其他基因的功能和表达，分析了它们的双突变体和三突变体的表型和Ca 2+特征以及它们的表达模式。
Similar to animals and plants, external stimuli cause dynamic spatial and temporal changes of cytoplasmic Ca2+ in fungi. Such changes are referred as the Ca2+ signature and control cellular responses by modulating the activity or location of diverse Ca2+-binding proteins (CBPs) and also indirectly affecting proteins that interact with CBPs. To understand the mechanism underpinning Ca2+ signaling, therefore, characterization of how Ca2+ moves to and from the cytoplasm to create Ca2+ signatures under different conditions is fundamental. Three genes encoding plasma membrane Ca2+ channels in a Fusarium graminearum strain that expresses a fluorescent protein-based Ca2+ indicator in the cytoplasm were mutagenized to investigate their roles in the generation of Ca2+ signatures under different growth conditions and genetic backgrounds. The genes disrupted include CCH1 and MIDI, which encode a high affinity Ca2+ uptake system, and FIG1, encoding a low affinity Ca2+ channel. Resulting mutants were also analyzed for growth, development, pathogenicity and mycotoxin production to determine how loss of each of the genes alters these traits. To investigate whether individual genes influence the function and expression of other genes, phenotypes and Ca2+ signatures of their double and triple mutants, as well as their expression patterns, were analyzed.