Rice OVATE family protein 6 regulates plant development and confers resistance to drought and cold stresses
Rice OVATE family protein 6 regulates plant development and confers resistance to drought and cold stresses
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水稻 OVATE 家族蛋白 6 调节植物发育并赋予对干旱和冷胁迫的抵抗力
DOI：
10.1093/jxb/erx309
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发表时间：
2017-08-01
影响因子：
6.9
通讯作者：
Li, Jianxiong
中科院分区：
文献类型：
--
作者：
Ma, Yamei;Yang, Chao;Li, Jianxiong
OVATE家族蛋白（OFPs）调节植物生长发育的多个方面，然而，它们在响应非生物胁迫中的作用尚未得到研究。在这里，我们报告水稻OsOFP 6调节生长和发育，并改变对干旱和寒冷胁迫的反应。OsOFP 6在不同组织和发育阶段表达。通过RNA干扰（RNAi）敲低OsOFP 6导致半矮秆身材、改变粒形和缩短侧根。OsOFP 6干扰生长素对侧根生长和发生的调控，因为RNAi植物在IAA（吲哚-3-乙酸）处理下侧根密度增加。OsOFP 6还影响生长素的极性运输，这通过RNAi和OsOFP 6过表达（OE）植物对N-1-萘基邻苯二甲酸（NPA）处理的不同反应来证明。此外，在干旱条件下，与RNAi植株相比，OE系表现出更慢的水分损失和更少的H2 O2积累，而RNAi植株表现出更快的水分损失和更高的H2 O2，这意味着OsOFP 6可能赋予水稻植物避免干旱和耐旱性。此外，低温处理下OE和RNAi植株的相对电导率（REC）分别降低和升高，表明OsOFP 6在低温胁迫下的响应中发挥重要作用。这些结果为OVATE家族蛋白的多种功能提供了新的见解。
OVATE family proteins (OFPs) regulate multiple aspects of plant growth and development; however, their roles in responses to abiotic stresses have not yet been studied. Here, we report that rice OsOFP6 regulates growth and development, and alters responses to drought and cold stresses. OsOFP6 is expressed in various tissues and developmental stages. Knock-down of OsOFP6 by RNA interference (RNAi) resulted in semi-dwarf stature, altered grain shape, and shorter lateral roots. OsOFP6 was shown to interfere with auxin in regulating lateral root growth and initiation, as RNAi plants had increased density of lateral roots under IAA (indole-3-acetic acid) treatment. OsOFP6 also affects polar auxin transport, which was demonstrated by the different responses of RNAi and OsOFP6-overexpression (OE) plants to treatment with N-1-naphthylphalamic acid (NPA). Furthermore, under drought conditions OE lines showed slower water loss and less accumulation of H2O2 compared with RNAi plants, which displayed faster water loss and higher H2O2, implying that OsOFP6 may confer both drought avoidance and drought tolerance in rice plants. In addition, under cold treatment OE and RNAi plants exhibited lower and higher relative electrical conductivity (REC), respectively, suggesting an important role of OsOFP6 in response to cold stress. These results provide new insights into the multiple functions of OVATE family proteins.