Purified mariner transposase is sufficient to mediate transposition in vitro
Purified mariner transposase is sufficient to mediate transposition in vitro
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DOI：
10.1002/j.1460-2075.1996.tb00930.x
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发表时间：
1996-10-01
期刊：
影响因子：
11.4
通讯作者：
Robertson, HM
中科院分区：
文献类型：
--
作者：
Lampe, DJ;Churchill, MEA;Robertson, HM
水手是一个广泛而多样的动物转座子家族。在三种不同的昆虫宿主物种的基因组中，存在着极其相似的刺激亚科分子，这强烈表明，物种特异性宿主因子对移动性是不必要的。我们通过检测角蝇中存在的这些元素之一（Himar1）的纯化转座酶的活性来验证这一假设。在体外质粒间转座反应中，Himar1转座酶足以忠实地复制转座。进一步分析表明，Himar1转座酶与其同源转座子的反向末端重复序列结合，并介导元件末端的5‘和3’剪切。物种特异性宿主因子的独立性有助于解释为什么水手具有如此广泛的分布以及为什么它们能够在物种之间水平转移。
Mariners are a widespread and diverse family of animal transposons. Extremely similar mariners of the irritans subfamily are present in the genomes of three divergent insect host species, which strongly suggests that species-specific host factors are unnecessary for mobility, We tested this hypothesis by examining the activity of a purified transposase from one of these elements (Himar1) present in the horn fly, Haematobia irritans. Himar1 transposase was sufficient to reproduce transposition faithfully in an in vitro inter-plasmid transposition reaction. Further analyses showed that Himar1 transposase binds to the Inverted terminal repeat sequences of its cognate transposon and mediates 5' and 3' cleavage of the element termini, Independence of species-specific host factors helps to explain why mariners have such a broad distribution and why they are capable of horizontal transfer between species.