Estrogen receptor α is involved in the regulation of ITGA8 methylation in estrogen receptor-positive breast cancer
Estrogen receptor α is involved in the regulation of ITGA8 methylation in estrogen receptor-positive breast cancer
复制标题
雌激素受体α参与雌激素受体阳性乳腺癌ITGA8甲基化的调节
DOI：
10.21037/atm-20-5220
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发表时间：
2020-08-01
影响因子：
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通讯作者：
Chen, Shuai
中科院分区：
文献类型：
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作者：
Wu, Jingxun;Cheng, Jianghong;Chen, Shuai
背景资料：整合素α 8亚基（ITGA 8）甲基化与几种癌症的发生有关，但其对乳腺癌的作用仍不清楚。本研究旨在探讨ITGA 8的甲基化状态，以及ITGA 8甲基化在乳腺癌中的潜在调控机制。方法：使用基因表达谱交互分析2（GEPLA 2）数据库和乳腺癌基因表达Miner v.4.4（bc-GenExMiner v4.4）研究ITGA 8的表达。使用癌症基因组图谱（TCGA）数据库和乳腺癌在线（GOBO）基于基因表达的结果：基因集分析评估ITGA 8表达水平与乳腺癌患者生存率之间的关联。甲基化特异性PCR（MSP）检测ITGA 8基因甲基化。结果：ITGA 8在人乳腺癌细胞中的表达低于非致瘤性乳腺细胞和乳腺组织，在雌激素受体（ER）阳性组织中的表达高于ER阴性组织（P
Background: Integrin subunit alpha 8 (ITGA8) methylation has been associated with the development of several cancers, but its contribution to breast cancer remains unclear. The present study aimed to investigate the methylation status of ITGA8, and the underlying regulatory mechanisms of ITGA8 methylation in breast cancer.Methods: ITGA8 expression was investigated using the Gene Expression Profiling Interactive Analysis 2 (GEPLA2) database and the Breast Cancer Gene-Expression Miner v.4.4 (bc-GenExMiner v4.4). The association between ITGA8 expression levels and the survival rate of breast cancer patients was evaluated using The Cancer Genomc Atlas (TCGA) database and Gene Expression-based Outcome for Breast Cancer Online (GOBO): Gene Set Analysis. Methylation-specific PCR (MSP) was used to detect the methylation of ITGA8. Protein level of ITGA8 was determined by Western blot analysis.Results: ITGA8 was expressed at low levels in human breast cancer cells compared to non-tumorigenic breast cells and breast tissue, and was upregulated in estrogen receptor (ER)-positive tissue compared with ER-negative tissue (P