Flow-induced hardening of endothelial nucleus as an intracellular stress-bearing organelle
Flow-induced hardening of endothelial nucleus as an intracellular stress-bearing organelle
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DOI：
10.1016/j.jbiomech.2005.06.003
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发表时间：
2005-09-01
影响因子：
2.4
通讯作者：
Sato, M
中科院分区：
文献类型：
--
作者：
Deguchi, S;Maeda, K;Sato, M
贴壁细胞中细胞核对承受胞内应力的机械作用尚不清楚。本文研究了流体剪切应力对内皮细胞核形态和弹性性能的影响。形态学观察表明，在静态条件下，细胞质中的细胞核呈垂直压缩状态，而在2 Pa （20 dyn/cm(2)）的流体剪切应力作用下，细胞核呈细长状，压缩程度更大。伸长的细胞核即使从细胞中分离出来后仍保持原来的形状。对离体核进行微管抽吸，结果显示，伸长核的弹性模量为0.62 +/- 0.15 kPa (n = 13，平均值+/- SD)，显著高于对照核的弹性模量0.42 +/- 0.12 kPa (n = 11)，表明细胞核在剪切应力作用下发生了结构重构。基于这些结果和透射电子显微镜，提出了细胞核作为细胞内承受压力的细胞器的可能性，这将影响对贴壁细胞中应力分布的解释。(C) 2005 Elsevier Ltd版权所有。
The mechanical contribution of nucleus in adherent cells to bearing intracellular stresses remains unclear. In this paper, the effects of fluid shear stress on morphology and elastic properties of endothelial nuclei were investigated. The morphological observation suggested that the nuclei in the cytoplasm were being vertically compressed under static conditions, whereas they were elongated and more compressed with a fluid shear stress of 2 Pa (20 dyn/cm(2)) onto the cell. The elongated nuclei remained the shape even after they were isolated from the cells. The micropipette aspiration technique on the isolated nuclei revealed that the elastic modulus of elongated nuclei, 0.62 +/- 0.15 kPa (n = 13, mean +/- SD), was significantly higher than that of control nuclei, 0.42 +/- 0.12 kPa (n = 11), suggesting that the nuclei remodeled their structure due to the shear stress. Based of these results and a transmission electron microscopy, a possibility of the nucleus as an intracellular compression-bearing organelle was proposed, which will impact interpretation of stress distribution in adherent cells. (C) 2005 Elsevier Ltd. All rights reserved.