Microtubule cycles in oocytes of the surf clam, Spisula solidissima: an immunofluorescence study.
Microtubule cycles in oocytes of the surf clam, Spisula solidissima: an immunofluorescence study.
复制标题
海蛤卵母细胞中的微管周期，Spisula Solidissima：一项免疫荧光研究。
DOI：
10.1016/0012-1606(86)90391-x
复制
发表时间：
1986
影响因子：
2.7
通讯作者：
Masui,Y
中科院分区：
文献类型：
--
作者：
Kuriyama,R;Borisy,GG;Masui,Y
Oocytes of the surf clam,Spisula solidissima, underwent germinal vesicle breakdown and two meiotic divisions to give off polar bodies when they were fertilized or parthenogenetically activated with KCl. Fertilized eggs further proceeded to mitosis and cleaved, while parthenogenetically activated eggs remained uncleaved. We examined changes in microtubule-containing structures during meiotic divisions and subsequent mitotic processes by immunofluorescence. A monoclonal anti-tubulin antibody was applied to alcohol-fixed eggs from which the vitelline membrane had been removed by protease digestion. Up to the stage of second polar body formation, the pattern of microtubule organization in the first and second meiotic spindles was identical in both fertilized and parthenogenetically activated eggs. However, while fertilized eggs formed a sperm aster and mitotic spindles later, activated eggs formed only monaster- or ring-shaped microtubule-containing structures which underwent cycles of alternating formation and breakdown. Lacto-orcein staining of parthenogenetically activated eggs revealed that the chromosome cycle could occur in these eggs, in phase with this microtubule cycle.
