A Novel Combination of Wheat Peptides and Fucoidan Attenuates Ethanol-Induced Gastric Mucosal Damage through Anti-Oxidant, Anti-Inflammatory, and Pro-Survival Mechanisms.
A Novel Combination of Wheat Peptides and Fucoidan Attenuates Ethanol-Induced Gastric Mucosal Damage through Anti-Oxidant, Anti-Inflammatory, and Pro-Survival Mechanisms.
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作者：
Kan J;Hood M;Burns C;Scholten J;Chuang J;Tian F;Pan X;Du J;Gui M
胃炎或消化性溃疡被认为影响到全球约一半的人。传统药物可能会导致不良反应，因此，需要替代的营养策略来防止胃粘膜损伤的发展。将小麦多肽和岩藻糖胶(WPF)制成两种食品级成分的新组合，在灌胃乙醇诱导大鼠胃粘膜损伤之前，对雄性SD大鼠进行30天的治疗。采用双抗体夹心酶联免疫吸附试验检测血清生物标志物水平。用免疫组织化学方法分析胃组织中的生物标志物。此外，以人胃上皮细胞系(GES-1)为材料，用Western印迹法检测其蛋白表达。胃复康口服液对乙醇所致的胃粘膜损伤有剂量依赖性逆转作用，溃疡指数和病理指数均有不同程度改善。WPF可提高超氧化物歧化酶水平，降低丙二醛水平。WPF还可降低白介素8、血小板活化因子和Caspase 3水平，升高前列腺素E-2、表皮生长因子(EGF)和EGF受体(EGFR)水平。此外，WPF还可诱导GES-1细胞中EGFR和细胞外信号调节蛋白激酶的磷酸化。总之，小麦多肽和岩藻糖胶的新组合通过抗氧化、抗炎和促进生存的机制减轻了乙醇引起的大鼠胃粘膜损伤。
Gastritis or peptic ulcer is believed to affect about half of people worldwide. Traditional medications can lead to adverse effects, therefore, alternative nutritional strategies are needed to prevent the development of gastric mucosal damage. A novel combination of two food-grade ingredients, wheat peptides and fucoidan (WPF), was prepared to treat male Sprague Dawley rats for 30 days before gastric mucosal damage was induced by oral administration of ethanol. The serum levels of biomarkers were determined by enzyme-linked immunosorbent assay. Biomarkers in stomach tissue were analyzed using immunohistochemistry. In addition, human gastric epithelial cell line (GES-1) was used to investigate protein expression by Western blot. WPF could attenuate ethanol-induced gastric mucosal damage in an inverse dose-dependent manner, with both ulcer index and pathological index improved. WPF increased superoxide dismutase level and decreased malondialdehyde level. WPF also decreased the levels of interleukin-8, platelet-activating factor, and Caspase 3, while increasing the levels of prostaglandin E-2, epidermal growth factor (EGF), and EGF receptor (EGFR). Furthermore, phosphorylation of EGFR and extracellular signal–regulated kinases was induced by WPF in GES-1 cells. In conclusion, the novel combination of wheat peptides and fucoidan attenuated ethanol-induced gastric mucosal damage in rats through anti-oxidant, anti-inflammatory, and pro-survival mechanisms.