Quality assessment of tissue specimens for studies of diabetic foot ulcers.
Quality assessment of tissue specimens for studies of diabetic foot ulcers.
复制标题
用于糖尿病足溃疡研究的组织标本的质量评估。
DOI：
10.1111/exd.12104
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发表时间：
2013
影响因子：
3.6
通讯作者：
Tomic-Canic,Marjana
中科院分区：
文献类型：
--
作者：
Stojadinovic,Olivera;Landon,JenniferN;Gordon,KatherineA;Pastar,Irena;Escandon,Julia;Vivas,Alejandra;Maderal,AndreaD;Margolis,DavidJ;Kirsner,RobertS;Tomic-Canic,Marjana
糖尿病足溃疡（DFU）是导致显著发病率和死亡率的重要临床问题。正在进行的转化研究努力更好地理解DFU病理学的分子/细胞基础，这可能导致识别新的治疗方案。未愈合伤口边缘的组织已被确定为DFU病理生理学的主要贡献者之一，为转化和临床研究提供了重要工具。为了评价组织标本的质量及其潜在用途，我们从25例患者中获得了81份DFU标本，并进行了组织学分析、免疫组化和RNA质量评估。我们发现，采集标本的深度是研究效用的重要决定因素，只有含有全层表皮的标本才能用于免疫组织化学和RNA分离。我们发现，只有三分之二的采集标本可用于转化研究。该损耗率对于涉及从DFU采集组织标本的未来研究的设计非常重要。
Diabetic foot ulcers (DFUs) represent an important clinical problem resulting in significant morbidity and mortality. Ongoing translational research studies strive to better understand molecular/cellular basis of DFU pathology that may lead to identification of novel treatment protocols. Tissue at the non‐healing wound edge has been identified as one of major contributors to the DFU pathophysiology that provides important tool for translational and clinical investigations. To evaluate quality of tissue specimens and their potential use, we obtained 81 DFU specimens from 25 patients and performed histological analyses, immunohistochemistry and RNA quality assessments. We found that depth of the collected specimen is important determinant of research utility, and only specimens containing a full‐thickness epidermis could be utilized for immunohistochemistry and RNA isolation. We showed that only two‐thirds of collected specimens could be utilized in translational studies. This attrition rate is important for designs of future studies involving tissue specimen collection from DFU.