Intranucleolar sites of ribosome biogenesis defined by the localization of early binding ribosomal proteins.
Intranucleolar sites of ribosome biogenesis defined by the localization of early binding ribosomal proteins.
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DOI：
10.1083/jcb.200612048
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发表时间：
2007-05-21
期刊：
影响因子：
--
通讯作者：
Scheer U
中科院分区：
文献类型：
--
作者：
Krüger T;Zentgraf H;Scheer U
人们正在进行相当大的努力来阐明核糖体生物发生的过程。虽然各种前核糖体RNP复合物已被分离和分子特征，核糖体蛋白（r-蛋白）除了新兴的核糖体亚基的顺序在很大程度上是未知的。此外，核糖体组装途径与专门的核糖体工厂（核仁）的结构组织之间的相关性还没有很好地建立。我们已经分析了几个早期结合r-蛋白在人类细胞的核仁定位，应用各种方法，包括活细胞成像和电子显微镜。我们已经将所有检测的r-蛋白（S4，S6，S7，S9，S14和L4）定位在颗粒组分（GC）中，这是核仁区域，随后的前核糖体RNA（rRNA）加工步骤发生。这些结果意味着，早期结合的r-蛋白不组装与新生的前rRNA转录在致密的纤维状组分（DFC），因为通常认为，并提供了r-蛋白组装和不同的颗粒在DFC-GC界面的出现之间的联系。
Considerable efforts are being undertaken to elucidate the processes of ribosome biogenesis. Although various preribosomal RNP complexes have been isolated and molecularly characterized, the order of ribosomal protein (r-protein) addition to the emerging ribosome subunits is largely unknown. Furthermore, the correlation between the ribosome assembly pathway and the structural organization of the dedicated ribosome factory, the nucleolus, is not well established. We have analyzed the nucleolar localization of several early binding r-proteins in human cells, applying various methods, including live-cell imaging and electron microscopy. We have located all examined r-proteins (S4, S6, S7, S9, S14, and L4) in the granular component (GC), which is the nucleolar region where later pre-ribosomal RNA (rRNA) processing steps take place. These results imply that early binding r-proteins do not assemble with nascent pre-rRNA transcripts in the dense fibrillar component (DFC), as is generally believed, and provide a link between r-protein assembly and the emergence of distinct granules at the DFC–GC interface.
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影响因子：
46.9
作者：
Nagai, T;Ibata, K;Miyawaki, A
通讯作者：
Miyawaki, A
影响因子：
14.9
作者：
Granneman, S;Gallagher, JEG;Pruijn, GJM
通讯作者：
Pruijn, GJM
影响因子：
16
作者：
Grandi, P;Rybin, V;Hurt, E
通讯作者：
Hurt, E
DOI：
10.1073/pnas.78.8.4823
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
CHOOI, WY;LEIBY, KR
通讯作者：
LEIBY, KR
DOI：
10.1073/pnas.83.19.7419
发表时间：
1986-10-01
影响因子：
11.1
作者：
ELKON, K;SKELLY, S;BROT, N
通讯作者：
BROT, N