The vaccine efficacy of recombinant duck enteritis virus expressing secreted E with or without PrM proteins of duck tembusu virus
The vaccine efficacy of recombinant duck enteritis virus expressing secreted E with or without PrM proteins of duck tembusu virus
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DOI：
10.1016/j.vaccine.2014.07.082
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发表时间：
2014-09-15
期刊：
影响因子：
5.5
通讯作者：
Chen, Hualan
中科院分区：
文献类型：
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作者：
Chen, Pucheng;Liu, Jinxiong;Chen, Hualan
自2010年以来，一种新出现的导致产蛋下降的登布苏病毒一直影响着中国的鸭子。目前还没有针对这种疾病的疫苗。自20世纪60年代以来，减毒活鸭肠炎病毒（DEV;一种疱疹病毒）疫苗已常规用于控制鸭中的致死性DEV。在这里，我们构建了两个重组DEV通过切除重叠fosmid DNA。一种病毒rDEV-TE表达鸭坦布苏病毒（DTMV）的包膜糖蛋白（TE）的截短形式，另一种病毒rDEV-PrM/TE同时表达TE和前膜蛋白（PrM）。动物实验表明，这两种重组病毒在鸭体内均能诱导出可测量的抗DTMUV中和抗体。在两次剂量的重组病毒后，rDEV-PrM/TE完全保护鸭免受DTMUV攻击，而rDEV-TE仅赋予部分保护。这些结果表明，表达TE和前膜蛋白的重组DEV是保护性的，可以作为一个潜在的候选疫苗，以防止DTMUV感染的鸭。(C)2014爱思唯尔有限公司版权所有。
A newly emerged tembusu virus that causes egg-drop has been affecting ducks in China since 2010. Currently, no vaccine is available for this disease. A live attenuated duck enteritis virus (DEV; a herpesvirus) vaccine has been used routinely to control lethal DEV in ducks since the 1960s. Here, we constructed two recombinant DEVs by transfecting overlapping fosmid DNAs. One virus, rDEV-TE, expresses the truncated form of the envelope glycoprotein (TE) of duck tembusu virus (DTMUV), and the other virus, rDEV-PrM/TE, expresses both the TE and pre-membrane proteins (PrM). Animal study demonstrated that both recombinant viruses induced measurable anti-DTMUV neutralizing antibodies in ducks. After two doses of recombinant virus, rDEV-PrM/TE completely protected ducks from DTMUV challenge, whereas rDEV-TE only conferred partial protection. These results demonstrate that recombinant DEV expressing the TE and pre-membrane proteins is protective and can serve as a potential candidate vaccine to prevent DTMUV infection in ducks. (C) 2014 Elsevier Ltd. All rights reserved.