Lamin B autoantibodies in sera of certain patients with systemic lupus erythematosus.
Lamin B autoantibodies in sera of certain patients with systemic lupus erythematosus.
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DOI：
10.1084/jem.165.3.750
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发表时间：
1987-03-01
期刊：
影响因子：
--
通讯作者：
Blobel G
中科院分区：
文献类型：
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作者：
Reeves WH;Chaudhary N;Salerno A;Blobel G
对四名系统性红斑狼疮患者的血清进行了鉴定和表征，其中含有抗体，这些抗体可通过间接免疫荧光对 HEp-2 细胞进行核边缘染色。每种血清都含有与蛋白质印迹中核纤层蛋白 B 发生强烈反应的自身抗体。四种血清中的一种与核纤层蛋白 A 和 C 表现出较弱的反应性，而其他三种血清仅与核纤层蛋白 A 和 C 表现出极小的反应性。抗核纤层蛋白抗体的滴度范围为 1:1,250 至 1:36,250。其中两份血清还以 1:20 的稀释度与 PTK-2 细胞的细胞质丝发生反应，表明这些血清中的一小部分自身抗体可能与核纤层蛋白的 α 螺旋结构域结合，该结构域与中间丝的结构同源。然而，这些患者血清中的大多数抗核纤层蛋白抗体对与核纤层蛋白 A 和 C 不同源的核纤层蛋白 B 分子部分具有特异性。研究结果表明，在某些情况下，核纤层的自身抗体可能是荧光抗核抗体测定中边缘图案的原因。此外，某些系统性红斑狼疮患者血清中针对核纤层的自身抗体可能有助于确定核纤层蛋白 B 的分子结构和生物学功能，以及研究自身免疫机制。
Sera from four patients with systemic lupus erythematosus containing antibodies that yield nuclear rim staining of HEp-2 cells by indirect immunofluorescence were identified and characterized. Each serum contained autoantibodies reacting strongly with lamin B on western blots. One of the four sera displayed weaker reactivity with lamins A and C, while the other three displayed only minimal reactivity with lamins A and C. Titers of antilamin antibodies ranged from 1:1,250 to 1:36,250. Two of the sera also reacted at a dilution of 1:20 with cytoplasmic filaments of PTK-2 cells, suggesting that a small fraction of the autoantibodies in these sera may bind to alpha-helical domains of the lamins that are homologous to those of intermediate filaments. The majority of the antilamin antibodies in these patients' sera are specific for portions of the lamin B molecule that are not homologous to lamins A and C, however. The findings suggest that autoantibodies to the nuclear lamina may, in some instances, be responsible for a rim pattern in the fluorescent antinuclear antibody assay. In addition, autoantibodies to the nuclear lamina in sera of certain patients with systemic lupus erythematosus may be useful for defining the molecular structure and biological functions of lamin B, as well as for studying mechanisms of autoimmunity.