INVITRO GROWTH-CHARACTERISTICS OF MELANOCYTES OBTAINED FROM ADULT NORMAL AND VITILIGO SUBJECTS
INVITRO GROWTH-CHARACTERISTICS OF MELANOCYTES OBTAINED FROM ADULT NORMAL AND VITILIGO SUBJECTS
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DOI：
10.1111/1523-1747.ep12469795
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发表时间：
1987-04-01
影响因子：
6.5
通讯作者：
RAMAIAH, A
中科院分区：
文献类型：
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作者：
PURI, N;MOJAMDAR, M;RAMAIAH, A
已研究从寻常性白癜风受试者未受累皮肤和病灶周围皮肤获得的黑素细胞的体外生长特征，并与健康成年供体的黑素细胞进行比较。已发现正常人黑素细胞在常规组织培养基中存在 10-11 M 霍乱毒素和 10 ng/ml 12-O-十四烷酰佛波醇-13-乙酸酯的情况下呈指数生长。它们最多可被胰蛋白酶消化 3-4 次。白癜风受试者未受累皮肤的黑素细胞的生长延迟了8-11天，并且无法传代。从色素沉着不足和色素沉着过度的病灶周围皮肤获得的黑素细胞在这些条件下均无法生长。只有在少数病灶周围皮肤正常色素沉着的情况下，黑素细胞才会在滞后 15 天后出现一些生长。白癜风患者未受累皮肤和病灶周围皮肤黑色素细胞的初始种植能力分别是正常人的50%和25%。白癜风病变本身会产生不明的树突状细胞，这些细胞可以存活 10-15 天，但没有表现出任何生长。我们的结果表明白癜风患者的黑素细胞有缺陷。任何关于白癜风病因的理论都必须考虑到这一事实。
The in vitro growth characteristics of melanocytes obtained from uninvolved and perilesional skin of vitiligo vulgaris subjects have been investigated in comparison to those from healthy adult donors. Normal human melanocytes have been found to grow exponentially in the presence of 10-11 M cholera toxin and 10 ng/ml of 12-O-tetradecanoylphorbol-13-acetate in routine tissue culture media. They could be trypsinized up to 3-4 passages. Melanocytes of the uninvolved skin of vitiligo subjects manifested a lag of 8-11 days for the onset of growth and they could not be passaged. Melanocytes obtained from both hypo- and hyperpigmented periolesional skin failed to grow under these conditions. Only in a few cases where the perilesional skin was normally pigmented did the melanocytes manifest some growth after a lag of 15 days. The initial seeding capacity of the melanocytes from uninvolved and perilesional skin of vitiligo patiens were, respectively, 50% and 25% of the normal individuals. Vitiligo lesions themselves gave rise to unidentified dendritic cells that survived for 10-15 days without manifesting any growth. Our results suggest that melanocytes of individuals with vitiligo are defective. This fact has to be taken into account in any theory on the etiology of vitiligo.