The Effects of 17β-estradiol on Mitochondrial Biogenesis and Function in Breast Cancer Cell Lines are Dependent on the ERα/ERβ Ratio
The Effects of 17β-estradiol on Mitochondrial Biogenesis and Function in Breast Cancer Cell Lines are Dependent on the ERα/ERβ Ratio
复制标题
DOI：
10.1159/000337607
复制
发表时间：
2012-01-01
影响因子：
--
通讯作者：
Roca, Pilar
中科院分区：
文献类型：
--
作者：
Sastre-Serra, Jorge;Nadal-Serrano, Mercedes;Roca, Pilar
背景/目的：17 β-雌二醇（E2）是乳腺癌发生的危险因素，可引起乳腺上皮细胞的肿瘤发生。此外，E2对不同组织具有不同的作用，这归因于两种雌激素受体亚型ER α和ER β的存在。研究方法：在两种具有不同雌激素受体比率的乳腺癌细胞系中研究了E2对线粒体生物发生和功能的影响，MCF-7（高ER α/ER β比率）和T47 D（低ER α/ER β比率）细胞系用生理浓度的E2（1 nM）处理。结果如下：MCF-7细胞系的线粒体显示增殖增加但功能降低，而具有低ER α/ER β比率的T47 D细胞系保持功能，线粒体较少。结论：我们的研究结果表明，ER的禀赋及其亚型关系对治疗反应有影响，并可能为乳腺癌中线粒体和ER的研究提供新的思路，以及疾病进展的新指标。版权所有（C）2012 S. Karger AG，巴塞尔
Background/Aims: 17 beta-estradiol (E2) is a risk factor for the development of breast cancer, and cause tumorigenesis in epithelial breast cells. Moreover, E2 has distinct effects on different tissues that are attributed to the presence of two estrogen receptor isoforms, ER alpha and ER beta. Methods: The effect of E2 on mitochondrial biogenesis and function was investigated in two breast cancer cell lines with different estrogen receptor ratios, MCF-7 (high ER alpha/ER beta ratio) and T47D (low ER alpha/ER beta ratio) cell lines treated with physiological concentrations of E2 (1 nM). Results: Mitochondria of the MCF-7 cell line showed an increase in proliferation but a decrease in functionality, while the T47D cell line, with low ER alpha/ER beta ratio, maintained functionality with fewer mitochondria. Conclusion: Our results suggest that ERs endowment and its subtypes relation have an effect on treatment response and could contribute new ideas about mitochondria and ERs in breast cancer, as well as new indicators to the disease progression. Copyright (C) 2012 S. Karger AG, Basel