Expression of heat shock protein 70a mRNA in Bombyx mori diapause eggs
Expression of heat shock protein 70a mRNA in Bombyx mori diapause eggs
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DOI：
10.1016/j.jinsphys.2010.03.023
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发表时间：
2010-09-01
影响因子：
2.2
通讯作者：
Yaginuma, Toshinobu
中科院分区：
文献类型：
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作者：
Moribe, Yoriko;Oka, Keigo;Yaginuma, Toshinobu
为了了解热休克蛋白70(Hsp70)是否参与了5℃环境下家蚕打破胚胎滞育的信号接收/转导，我们克隆了Hsp70a的cDNA，并用实时定量聚合酶链式反应检测了Hsp70a在家蚕滞育卵和非滞育卵中的表达。在产卵后持续保持在25℃的滞育卵中，Hsp70a的mRNA逐渐增加，以维持滞育。当滞育卵从产卵后第2天开始暴露在5℃的滞育终止条件下时，Hsp70a mRNA在冷处理后第5天开始增加。即使在非滞育卵中，Hsp70a的mRNA在5℃时也略有增加。这些结果表明，Hsp70a通过基因激活参与滞育终止(5℃)信号的接收/转导。结合冷反应基因Samui的表达模式对Hsp70a基因的表达模式进行了讨论。(C)2010爱思唯尔有限公司。保留所有权利。
In an effort to understand whether heat shock protein 70 (Hsp70) participates in the environmental 5 degrees C signal reception/transduction toward breaking embryonic diapause of the silkworm Bombyx mori, we isolated a cDNA for Hsp70a and examined the expression of Hsp70a mRNA in B. mori diapause and nondiapause eggs by quantitative real-time PCR. Hsp70a mRNA gradually increased in diapause eggs continuously kept at 25 degrees C after oviposition to maintain diapause. When diapause eggs were exposed to the diapause-terminating condition of 5 degrees C beginning at 2 days post-oviposition, Hsp70a mRNA increased beginning at 5 days post-cold treatment. Even in nondiapause eggs, Hsp70a mRNA increased slightly with exposure to 5 degrees C. These results suggest that Hsp70a is involved in reception/transduction of the diapause-terminating (5 degrees C) signal via gene activation. The expression patterns of Hsp70a mRNA are discussed in relation to those of the cold-response gene Samui. (C) 2010 Elsevier Ltd. All rights reserved.