Comparative genomic analysis in the region of a major Plasmodium-refractoriness locus of Anopheles gambiae.
Comparative genomic analysis in the region of a major Plasmodium-refractoriness locus of Anopheles gambiae.
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冈比亚按蚊主要疟原虫难治基因座区域的比较基因组分析。
DOI：
10.1073/pnas.082235599
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Collins,FrankH
中科院分区：
文献类型：
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作者：
Thomasova,Dana;Ton,LucasQ;Copley,RichardR;Zdobnov,EvgenyM;Wang,Xuelan;Hong,YoungS;Sim,Cheolho;Bork,Peer;Kafatos,FotisC;Collins,FrankH
我们对来自冈比亚按蚊（非洲人类疟疾的主要媒介）实验室菌株的细菌人工染色体（BAC）克隆文库中的6个重叠克隆进行了测序。由此得到的528kb的不间断序列来自于蚊子染色体的8g区域，位于或非常接近与寄生虫黑素包被相关的主要难降解位点。该序列代表了包含48个基因的蚊子基因组结构的第一个广泛视图。基因组比较表明，大部分同源基因存在于黑腹果蝇的6个微同质群中。一个完全包含在该区域内的BAC克隆表明该物种存在显著程度的局部多态性，这可能对其种群结构和媒介能力具有重要意义。
We have sequenced six overlapping clones from a library of bacterial artificial chromosome (BAC) clones derived from a laboratory strain of the mosquito,Anopheles gambiae, the major vector of human malaria in Africa. The resulting uninterrupted 528-kb sequence is from the8Cregion of the mosquito2Rchromosome, at or very near the major refractoriness locus associated with melanotic encapsulation of parasites. This sequence represents the first extensive view of the mosquito genome structure encompassing 48 genes. Genomic comparison reveals that the majority of the orthologues are found in six microsyntenic clusters inDrosophila melanogaster. A BAC clone that is wholly contained within this region demonstrates the existence of a remarkable degree of local polymorphism in this species, which may prove important for its population structure and vectorial capacity.