Ca2+ and CaM kinase regulate neurofilament expression.
Ca2+ and CaM kinase regulate neurofilament expression.
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Ca2 和 CaM 激酶调节神经丝表达。
DOI：
10.1097/00001756-200311140-00013
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发表时间：
2003
期刊：
影响因子：
1.7
通讯作者：
Vallano,MaryL
中科院分区：
文献类型：
--
作者：
Bui,CuongJ;Beaman-Hall,CarolM;Vallano,MaryL
用培养的小脑颗粒神经元验证了NMDA或KCl依赖的钙内流激活了介导神经丝转录、翻译和组装的信号级联的假说。结果表明，2 5 mM KCl1和1 40μMNMDa可诱导神经突起内神经丝蛋白、基因和组装，并分别需要通过电压敏感的钙通道或NMDA型受体进入神经纤维。CaM激酶和转录抑制剂KN-62和放线菌素D分别减弱了钙的刺激作用。颗粒神经元是探索神经元活动对神经丝基因转录调控的分子基础的有价值的模型。
Cultured cerebellar granule neurons were used to examine the hypothesis that NMDA or KCl-dependent Ca 2+ influx activates a signaling cascade mediating transcription, translation and assembly of neurofilaments. The data indicate that 25 mM KCl and 140 μM NMDA induce neurofilament protein, mRNA, and assembly in neurites, and these require Ca 2+ entry through voltage-sensitive Ca 2+ channels or NMDA receptors, respectively. The CaM kinase and transcriptional inhibitors, KN-62 and actinomycin D, respectively, attenuate the stimulatory effect of Ca 2+. Granular neurons are a valuable model to explore the molecular basis for transcriptional regulation of neurofilament gene expression by neuronal activity.