The regulation of tooth morphogenesis is associated with epithelial cell proliferation and the expression of Sonic hedgehog through epithelial-mesenchymal interactions
The regulation of tooth morphogenesis is associated with epithelial cell proliferation and the expression of Sonic hedgehog through epithelial-mesenchymal interactions
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DOI：
10.1016/j.bbrc.2011.01.052
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发表时间：
2011-02-18
影响因子：
3.1
通讯作者：
Tsuji, Takashi
中科院分区：
文献类型：
--
作者：
Ishida, Kentaro;Murofushi, Mayumi;Tsuji, Takashi
外胚层器官，如牙齿、唾液腺、毛发和乳腺，通过相互的上皮-间充质相互作用而发育。牙齿形态由牙冠宽度和牙齿长度定义(宏观形态)，由牙尖和牙根的数量和位置定义(微观形态)。在我们目前的研究中，我们报道了用分离的上皮细胞和间充质细胞通过器官胚芽方法重建的生物工程磨牙的牙冠宽度可以通过上皮细胞和间充质细胞层之间的接触面积来调节。我们进一步证明，这与细胞增殖和Sonic hedgehog(Shh)在胚胎期形成次级釉质结后内釉质上皮中的表达有关。我们还证明了牙尖数目与生物工程牙齿的牙冠宽度显著相关。这些发现表明，牙齿的微观形态，即牙尖的形成，是在确定牙齿宽度或宏观形态之后进行调节的。这些发现还表明，细胞增殖的时空模式和上皮中Shh的表达区域调节着发育中牙齿的牙冠宽度和牙尖形成。(C)2011 Elsevier Inc.保留所有权利。
Ectodermal organs, such as the tooth, salivary gland, hair, and mammary gland, develop through reciprocal epithelial-mesenchymal interactions. Tooth morphologies are defined by the crown width and tooth length (macro-morphologies), and by the number and locations of the cusp and roots (micro-morphologies). In our current study, we report that the crown width of a bioengineered molar tooth, which was reconstructed using dissociated epithelial and mesenchymal cells via an organ germ method, can be regulated by the contact area between epithelial and mesenchymal cell layers. We further show that this is associated with cell proliferation and Sonic hedgehog (Shh) expression in the inner enamel epithelium after the germ stage has formed a secondary enamel knot. We also demonstrate that the cusp number is significantly correlated with the crown width of the bioengineered tooth. These findings suggest that the tooth micro-morphology, i.e. the cusp formation, is regulated after the tooth width, or macro-morphology, is determined. These findings also suggest that the spatiotemporal patterning of cell proliferation and the Shh expression areas in the epithelium regulate the crown width and cusp formation of the developing tooth. (C) 2011 Elsevier Inc. All rights reserved.