Culture of mouse embryonic stem cells on photoimmobilized polymers
Culture of mouse embryonic stem cells on photoimmobilized polymers
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DOI：
10.1263/jbb.102.304
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发表时间：
2006-10-01
影响因子：
2.8
通讯作者：
Ito, Yoshihiro
中科院分区：
文献类型：
--
作者：
Konno, Tomohiro;Kawazoe, Naoki;Ito, Yoshihiro
小鼠胚胎干 (ES) 细胞在具有不同表面特性的四种聚合物上培养。这些聚合物是聚（丙烯酸）、聚烯丙胺、明胶和聚（2-甲基丙烯酰氧基乙基磷酰胆碱-共-甲基丙烯酸）（PMAc50），并与叠氮基苯基偶联并光固定在常规聚苯乙烯细胞培养皿上。在固定化聚合物表面培养小鼠ES细胞，观察细胞形态、细胞生长、碱性磷酸酶染色、转录因子stat3的激活以及八聚体结合蛋白3/4（Oct3/4）转录因子和含锌指转录因子（GATA4）的表达。形态和生长速率受到聚合物表面性质的显着影响。 ES细胞附着在明胶或聚烯丙胺表面；然而，在前者上形成了殖民地，而在后者上则没有。此外，在明胶表面观察到生长的显着增强。相反，ES细胞在光固定聚丙烯酸表面和PMAc50表面聚集形成拟胚体，尽管细胞生长减少。在形态学和基因表达分析中观察到 ES 细胞在 PMAc50 表面上的聚集显着增强。
Mouse embryonic stem (ES) cells were cultured on four types of polymer with different surface properties. The polymers were poly(acrylic acid), polyallylamine, gelatin, and poly(2-methacryloyloxyethyl phosphoryleholine-co-methacrylic acid) (PMAc50), and were coupled with azidophenyl groups and photoimmobilized on conventional polystyrene cell-culture dishes. Mouse ES cells were cultured on the immobilized polymer surfaces, and cell morphology, cell growth, staining for alkaline phosphatase, activation of the transcription factor stat3, and expression of the octamer-binding protein 3/4 (Oct3/4) transcription factor and the zinc finger-containing transcription factor (GATA4) were observed. Morphology and growth rate were significantly affected by the polymer surface properties. The ES cells attached to gelatin or polyallylamine surfaces; however, colonies formed on the former but not the latter. In addition, significant enhancement of growth was observed on the gelatin surface. In contrast, ES cells aggregated to form an embryoid body on the photoimmobilized poly(acrylic acid) surface and the PMAc50 surface, although cell growth was reduced. Significant enhancement of aggregation of ES cells on the PMAc50 surface was observed in morphology and gene expression analyses.