DNA molecules and configurations in a solid-state nanopore microscope
DNA molecules and configurations in a solid-state nanopore microscope
复制标题
DOI：
10.1038/nmat965
复制
发表时间：
2003-09-01
期刊：
影响因子：
41.2
通讯作者：
Golovchenko, JA
中科院分区：
文献类型：
--
作者：
Li, JL;Gershow, M;Golovchenko, JA
固态膜中的纳米级孔提供了一种电子探测单一线性聚合物结构的新方法，包括那些在其天然环境中具有生物学意义的聚合物。以前的工作与生物蛋白质孔足够宽，让通过和传感单链DNA分子展示了使用纳米孔的力量，但许多未来的任务;和应用程序需要一个强大的固态孔，其纳米尺度的尺寸和属性可以选择，作为一个选择显微镜的镜头。在这里，我们展示了一种固态纳米孔显微镜，能够观察双链DNA的单个分子及其折叠行为。我们讨论了纳米孔显微镜概念的扩展，以替代探测机制和应用，包括分子结构和测序的研究。
A nanometre-scale pore in a solid-state membrane provides a new way of electronically probing the structure of single linear polymers, including those of biological interest in their native environments. Previous work with biological protein pores wide enough to let through and sense single-stranded DNA molecules demonstrates the power of using nanopores, but many future tasks; and applications call for a robust solid-state pore whose nanometre-scale dimensions and properties may be selected, as one selects the lenses of a microscope. Here we demonstrate a solid-state nanopore microscope capable of observing individual molecules of double-stranded DNA and their folding behaviour. We discuss extensions of the nanopore microscope concept to alternative probing mechanisms and applications, including the study of molecular structure and sequencing.