Protein tyrosine phosphatase receptor-like genes are frequently hypermethylated in sporadic colorectal cancer
Protein tyrosine phosphatase receptor-like genes are frequently hypermethylated in sporadic colorectal cancer
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DOI：
10.1038/jhg.2012.119
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发表时间：
2013-01-01
影响因子：
3.5
通讯作者：
Sasiadek, Maria M.
中科院分区：
文献类型：
--
作者：
Laczmanska, Izabela;Karpinski, Pawel;Sasiadek, Maria M.
磷酸酶的活性可能受到遗传和表观遗传改变的影响。在我们的研究中，我们研究了四种ptpr: PTPRM、PTPRT、PTPRR和PTPRZ1的甲基化状态，这些ptpr是通过微阵列技术预先选择的，在散发性结直肠癌（CRC）中被甲基化。对131例散发性结直肠癌患者的手术标本进行分析。采用甲基特异性PCR （MSP）检测这4个基因的甲基化状态。利用Illumina 27K芯片分析启动子甲基化，发现4个蛋白酪氨酸磷酸酶PTPRM、PTPRT、PTPRR和PTPRZ1被超甲基化，β值>= 0.2和P
The activity of phosphatases could be influenced by genetic, as well as epigenetic alterations. In our study, we have investigated the methylation status of four PTPRs: PTPRM, PTPRT, PTPRR and PTPRZ1, which were pre-selected using microarray techniques as being alternatively methylated in sporadic colorectal cancer (CRC). The analyses were carried out on 131 surgical specimens obtained from sporadic CRC patients. The methylation status of the four genes was examined using methyl specific PCR (MSP). The analysis of promoter methylation using an Illumina 27K microarray revealed four protein tyrosine phosphatases PTPRM, PTPRT, PTPRR and PTPRZ1 as being hypermethylated with beta-value >= 0.2 and P