Warm temperatures activate TRPV4 in mouse 308 keratinocytes
Warm temperatures activate TRPV4 in mouse 308 keratinocytes
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DOI：
10.1074/jbc.m303251200
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发表时间：
2003-08-22
影响因子：
4.8
通讯作者：
Caterina, MJ
中科院分区：
文献类型：
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作者：
Chung, MK;Lee, H;Caterina, MJ
哺乳动物的生存需要不断监测环境和体温。最近，瞬时受体电位香草蛋白（TRPV）离子通道亚家族的几个成员已经被确定，可以通过温度升高进入温暖（TRPV3和TRPV4）或痛苦热（TRPV1和TRPV2）范围。在啮齿动物中，TRPV3和TRPV4蛋白未在感觉神经元中检测到，但在皮肤表皮角质形成细胞中高度表达。在这里，我们表明，在响应温暖的温度（bbb32℃），小鼠308角质形成细胞系表现出非选择性的跨膜阳离子电流和Ca2+内流。TRPV3和TRPV4均在308个细胞中表达。然而，我们观察到的热诱发反应与重组TRPV4介导的反应最相似，这是基于它们的电生理特性和对渗透压和苯酚酯（4 α -苯酚-12,13-二decanoate）的敏感性。总之，这些数据支持了角质形成细胞能够检测适度温度升高的观点，强烈表明TRPV4参与了这些反应，并定义了一个温度转导的细胞生物学分析系统。
Mammalian survival requires constant monitoring of environmental and body temperature. Recently, several members of the transient receptor potential vanilloid (TRPV) subfamily of ion channels have been identified that can be gated by increases in temperature into the warm (TRPV3 and TRPV4) or painfully hot (TRPV1 and TRPV2) range. In rodents, TRPV3 and TRPV4 proteins have not been detected in sensory neurons but are highly expressed in skin epidermal keratinocytes. Here, we show that in response to warm temperatures (>32degreesC), the mouse 308 keratinocyte cell line exhibits nonselective transmembrane cationic currents and Ca2+ influx. Both TRPV3 and TRPV4 are expressed in 308 cells. However, the warmth-evoked responses we observe most closely resemble those mediated by recombinant TRPV4 on the basis of their electrophysiological properties and sensitivity to osmolarity and the phorbol ester, 4alpha-phorbol-12,13-didecanoate. Together, these data support the notion that keratinocytes are capable of detecting modest temperature elevations, strongly suggest that TRPV4 participates in these responses, and define a system for the cell biological analysis of warmth transduction.