Biomimetic mineralization using matrix vesicle nanofragments
Biomimetic mineralization using matrix vesicle nanofragments
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DOI：
10.1002/jbm.a.36618
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发表时间：
2019-05-01
影响因子：
4.9
通讯作者：
Matsumoto, Takuya
中科院分区：
文献类型：
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作者：
Kunitomi, Yosuke;Hara, Emilio Satoshi;Matsumoto, Takuya
近年来，骨组织的体外合成受到关注；然而，由于在对体内真实骨形成过程的理解以及在骨合成中知识的应用方面仍存在一些差距，目前制造骨组织的方法仍然效果不佳。因此，本研究的目的首先是从材料科学家的角度对小鼠颅骨膜内成骨过程中初始矿物质形成进行系统的超微结构研究，并基于体内研究结果开发新的矿化方法。首先，发现小鼠颅骨中最初的矿物质沉积发生在胚胎第E14.0天。对初始骨形成过程的分析表明，它涉及以下不同步骤：胶原蛋白分泌、基质小泡（MV）释放、MV矿化、MV破裂以及胶原纤维矿化。接下来，我们使用MV和水凝胶支架进行了体外矿化实验。包埋在胶原凝胶中的完整MV没有矿化，而有趣的是，通过超声处理获得的MV纳米碎片能够促进快速矿化。这些结果表明，机械破裂的MV膜可能是一种有前途的体外骨组织合成材料。（c）2019作者。《生物医学材料研究杂志A部分》由威利期刊公司出版。《生物医学材料研究杂志A部分》：107A：1021 - 1030，2019年。
In vitro synthesis of bone tissue has been paid attention in recent years; however, current methods to fabricate bone tissue are still ineffective due to some remaining gaps in the understanding of real in vivo bone formation process, and application of the knowledge in bone synthesis. Therefore, the objectives of this study were first, to perform a systematic and ultrastructural investigation of the initial mineral formation during intramembranous ossification of mouse calvaria from a material scientists' viewpoint, and to develop novel mineralization methods based on the in vivo findings. First, the very initial mineral deposition was found to occur at embryonic day E14.0 in mouse calvaria. Analysis of the initial bone formation process showed that it involved the following distinct steps: collagen secretion, matrix vesicle (MV) release, MV mineralization, MV rupture, and collagen fiber mineralization. Next, we performed in vitro mineralization experiments using MVs and hydrogel scaffolds. Intact MVs embedded in collagen gel did not mineralize, whereas, interestingly, MV nanofragments obtained by ultrasonication could promote rapid mineralization. These results indicate that mechanically ruptured MV membrane can be a promising material for in vitro bone tissue synthesis. (c) 2019 The Authors. journal Of Biomedical Materials Research Part A Published By Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1021-1030, 2019.