The NineTeen Complex (NTC) and NTC-associated proteins as targets for spliceosomal ATPase action during pre-mRNA splicing.
The NineTeen Complex (NTC) and NTC-associated proteins as targets for spliceosomal ATPase action during pre-mRNA splicing.
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DOI：
10.1080/15476286.2015.1008926
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发表时间：
2015
期刊：
影响因子：
4.1
通讯作者：
O'Keefe RT
中科院分区：
文献类型：
--
作者：
de Almeida RA;O'Keefe RT
Pre-mRNA剪接是基因表达的一个重要步骤，它可以有效、准确地去除内含子序列，从而产生成熟的mRNA进行翻译。它是一种叫做剪接体的大而动态的rna -蛋白质复合物，催化内含子的去除。为了进行剪接，剪接体不仅需要与前体mrna正确组装，而且剪接体需要对其RNA和蛋白质成分进行广泛的重构，以执行内含子移除的两个步骤。剪接体的重塑是通过atp酶的作用来实现的，atp酶以RNA和蛋白质为目标，产生剪接体的构象，这些构象能够完成剪接体激活、催化和拆卸的每一步。越来越多的研究指出，剪接体相关的19复合体（NTC）蛋白是剪接体重塑过程中许多atp酶作用的靶标。在这篇观点文章中，我们介绍了剪接体激活、催化和拆卸所需的atp酶作用后NTC发生变化的最新发现。我们提出NTC是ATPase在pre-mRNA剪接所需的剪接体重塑过程中的主要作用靶点之一。
Pre-mRNA splicing is an essential step in gene expression that removes intron sequences efficiently and accurately to produce a mature mRNA for translation. It is the large and dynamic RNA-protein complex called the spliceosome that catalyzes intron removal. To carry out splicing the spliceosome not only needs to assemble correctly with the pre-mRNA but the spliceosome requires extensive remodelling of its RNA and protein components to execute the 2 steps of intron removal. Spliceosome remodelling is achieved through the action of ATPases that target both RNA and proteins to produce spliceosome conformations competent for each step of spliceosome activation, catalysis and disassembly. An increasing amount of research has pointed to the spliceosome associated NineTeen Complex (NTC) of proteins as targets for the action of a number of the spliceosomal ATPases during spliceosome remodelling. In this point-of-view article we present the latest findings on the changes in the NTC that occur following ATPase action that are required for spliceosome activation, catalysis and disassembly. We proposed that the NTC is one of the main targets of ATPase action during spliceosome remodelling required for pre-mRNA splicing.